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Literature summary for 1.1.1.47 extracted from

  • Makino, Y.; Negoro, S.; Urabe, I.; Okada, H.
    Stability-increasing mutants of glucose dehydrogenase from Bacillus megaterium IWG3 (1989), J. Biol. Chem., 264, 6381-6385.
    View publication on PubMed
Search for more literature for 1.1.1.47

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Priestia megaterium

Protein Variants

Protein Variants Comment Organism
E96A mutant enzyme has higher stability at 60°C and 90% remaining activity compared to the wild type enzyme Priestia megaterium
E96G mutant enzyme has higher stability at 60°C and 47% remaining activity compared to the wild type enzyme Priestia megaterium
E96K/D108N/P194Q/E210K mutant enzyme has higher stability at 60°C and 97% remaining activity compared to the wild type enzyme Priestia megaterium
E96K/V112A/E133K/Y217H mutant enzyme has higher stability at 60°C and 85% remaining activity compared to the wild type enzyme Priestia megaterium
E96K/V183I mutant enzyme has higher stability at 60°C and 72% remaining activity compared to the wild type enzyme Priestia megaterium
Q252L mutant enzyme has higher stability at 60°C and 56% remaining activity compared to the wild type enzyme Priestia megaterium
Q252L/A258G mutant enzyme has higher stability at 60°C and 61% remaining activity compared to the wild type enzyme Priestia megaterium
Y253C mutant enzyme has higher stability at 60°C and 1% remaining activity compared to the wild type enzyme Priestia megaterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28090
-
 subunit, calculated from amino acid sequence Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium P40288
-
-
Priestia megaterium IWG3 P40288
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephadex A-50 gel filtration Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + NAD+
-
 Priestia megaterium D-glucono-1,5-lactone + NADH + H+
-
 ?
D-glucose + NAD+
-
 Priestia megaterium IWG3 D-glucono-1,5-lactone + NADH + H+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Priestia megaterium