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Literature summary for 1.1.1.47 extracted from

  • Thompson, R.E.; Carper, W.R.
    Glucose dehydrogenase from pig liver. I. Isolation and purification (1970), Biochim. Biophys. Acta, 198, 397-406.
    View publication on PubMed
Search for more literature for 1.1.1.47

Crystallization (Commentary)

Crystallization (Comment) Organism
precipitate is dissolved in deionized water and ammonium sulfate added to 23% saturation Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Sus scrofa
Fe2+
-
 Sus scrofa
PCMB
-
 Sus scrofa
Zn2+
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
 NAD+ pH 9.9, 30°C Sus scrofa
70
-
 beta-D-glucose pH 9.9, 30°C Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, acetic acid pH 5.5, Sephadex G200, G150, crystallization Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.27
-
-
 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + NAD+
-
 Sus scrofa D-glucono-1,5-lactone + NADH + H+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Sus scrofa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 48 15°C: about 35% of maximal activity, 48°C: 60% of maximal activity Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.9
-
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
8 11 about 45% of maximal activity at pH 8.0 and pH 11.0 Sus scrofa