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Literature summary for 1.1.1.47 extracted from
- Reactivation of inactivated D-glucose dehydrogenase of a Bacillus species by pyridine and adenine nucleotides (1979), Agric. Biol. Chem., 43, 271-278.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ADP | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) |  |
| AMP | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
| ATP | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
| dipicolinic acid | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
| NAD+ | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
| NADH | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
| NADP+ | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
| NADPH | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
General Stability
| General Stability | Organism |
|---|---|
| crude enzyme loses 90% activity after 3 days at 5°C | Bacillus sp. (in: Bacteria) |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.061 | - |
NADP+ | - |
Bacillus sp. (in: Bacteria) | |
| 0.082 | - |
NAD+ | - |
Bacillus sp. (in: Bacteria) | |
| 4.2 | - |
beta-D-glucose | - |
Bacillus sp. (in: Bacteria) | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
| KCl | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
| Na2SO4 | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
| NaCl | NAD+, NADH, NADP+, NADPH, AMP, ADP, ATP, dipicolinic acid, (NH4)2SO4, Na2SO4, NaCl and KCl reactivate partially purified enzyme inactivated by storage at 4°C, optimal reactivation at 30°C and pH 7.0, enzyme may be regulated by monomer-dimer interconversion | Bacillus sp. (in: Bacteria) | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
inactive enzyme form, gel filtration | Bacillus sp. (in: Bacteria) |
| 80000 | - |
active enzyme form, gel filtration | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
D-ribose producing mutant | - |
| Bacillus sp. (in: Bacteria) BG 1722 | - |
D-ribose producing mutant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose, partially purified enzyme, 10 mM NAD(P)+ protects from inactivation on column | Bacillus sp. (in: Bacteria) |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 89% loss of activity after 4 days without stabilzing agent, complete stabilization by 10 mM NAD+ or 10 mM NADP+, and to a lesser extent by 800 mM NaCl. Partial stabilization by 800 MM (NH4)2SO4, 800 mM Na2SO4 or 800 mM dipicolinic acid | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-D-glucose + NAD+ | 113% of the activity with D-glucose | Bacillus sp. (in: Bacteria) | 2-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
| 2-deoxy-D-glucose + NAD+ | 113% of the activity with D-glucose | Bacillus sp. (in: Bacteria) BG 1722 | 2-deoxy-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
| 2-deoxy-D-glucose + NADP+ | 103% of the activity with D-glucose and NAD+ | Bacillus sp. (in: Bacteria) | 2-deoxy-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
| 2-deoxy-D-glucose + NADP+ | 103% of the activity with D-glucose and NAD+ | Bacillus sp. (in: Bacteria) BG 1722 | 2-deoxy-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
| beta-D-glucose + NADP+ | 93% of the activity with NAD+ | Bacillus sp. (in: Bacteria) | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
| beta-D-glucose + NADP+ | 93% of the activity with NAD+ | Bacillus sp. (in: Bacteria) BG 1722 | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
| maltose + NAD+ | 6% of the activity with D-glucose | Bacillus sp. (in: Bacteria) | ? + NADH | - |
? | |
| maltose + NAD+ | 6% of the activity with D-glucose | Bacillus sp. (in: Bacteria) BG 1722 | ? + NADH | - |
? | |
| maltose + NADP+ | 5% of the activity D-glucose and NAD+ | Bacillus sp. (in: Bacteria) | ? + NADPH | - |
? | |
| maltose + NADP+ | 5% of the activity D-glucose and NAD+ | Bacillus sp. (in: Bacteria) BG 1722 | ? + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | - |
Bacillus sp. (in: Bacteria) |
| tetramer | 2 * 45000, gel filtration of inactive monomeric enzyme | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Bacillus sp. (in: Bacteria) |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Bacillus sp. (in: Bacteria) | |
| NADP+ | - |
Bacillus sp. (in: Bacteria) | |
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