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Literature summary for 1.1.1.44 extracted from

  • Wang, Y.; Percival, Y.
    Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration (2009), Microb. Cell Fact., 8, 30 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.44

Application

Application Comment Organism
synthesis NADPH regeneration. When coupled with glucose-6-phosphate dehydrogenase the enzyme generates two moles of NADPH per mole of glucose-6-phosphate Thermotoga maritima

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NADP+ 80°C, pH 7.5 Thermotoga maritima
0.011
-
 6-phospho-D-gluconate 80°C, pH 7.5 Thermotoga maritima
0.38
-
 NAD+ 80°C, pH 7.5 Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WYR9
-
-
Thermotoga maritima DSM 3109 Q9WYR9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NAD+ the enzyme prefers NADP+ to NAD+ as an electron acceptor. Km-value for NAD+ is 38fold higher than the Km-value for NADP+ Thermotoga maritima D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
6-phospho-D-gluconate + NAD+ the enzyme prefers NADP+ to NAD+ as an electron acceptor. Km-value for NAD+ is 38fold higher than the Km-value for NADP+ Thermotoga maritima DSM 3109 D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
6-phospho-D-gluconate + NADP+ the enzyme preferrs NADP+ to NAD+ as an electron acceptor Thermotoga maritima D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
6-phospho-D-gluconate + NADP+ the enzyme preferrs NADP+ to NAD+ as an electron acceptor Thermotoga maritima DSM 3109 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
6PGDH
-
 Thermotoga maritima
TM0438
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 95 70°C: about 55% of maximal activity, 95°C: about 65% of maximal activity Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 half-life: 140 h Thermotoga maritima
90
-
 half-life: 48 h Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
325
-
 NADP+ 80°C, pH 7.5 Thermotoga maritima
325
-
 6-phospho-D-gluconate 80°C, pH 7.5 Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
6 9 about 70% of the activity remains at pH 6.0 and 9.0 Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
NADP+ the enzyme preferrs NADP+ to NAD+ as an electron acceptor Thermotoga maritima

Expression

Organism Comment Expression
Thermotoga maritima through numerous approaches including expression vectors, hosts, cultivation conditions, inducers, and codon-optimization of the 6pgdh gene, the soluble 6PGDH expression levels are enhanced to about 250 mg per liter of culture by more than 500fold up