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Literature summary for 1.1.1.44 extracted from

  • Chen, Y.Y.; Ko, T.P.; Chen, W.H.; Lo, L.P.; Lin, C.H.; Wang, A.H.
    Conformational changes associated with cofactor/substrate binding of 6-phosphogluconate dehydrogenase from Escherichia coli and Klebsiella pneumoniae: Implications for enzyme mechanism (2010), J. Struct. Biol., 169, 25-35.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the A134T mutant is expressed in Escherichia coli BL21 (DE3) cells Klebsiella pneumoniae
the Y378C mutant is expressed in Escherichia coli BL21 (DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
complexed with substrate, substrate/NADPH and glucose, sitting drop vapour diffusion method, at 4°C, with 0.12 M diammonium hydrogen citrate buffer (pH 5.0) and 20% (w/v) polyethylene glycol (PEG) 3350 Klebsiella pneumoniae
complexed with substrate, substrate/NADPH and glucose, sitting drop vapour diffusion method, using 0.1 M sodium citrate dihydrate buffer (pH5.4), 0.5 M ammonium acetate, 6-7% (w/v) PEG 3350, and 17-18% (w/v) PEG 4000 as a reservoir, at 18°C Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.049
-
NADP+ in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25°C Escherichia coli
0.093
-
6-phospho-D-gluconate in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25°C Escherichia coli
0.107
-
6-phospho-D-gluconate in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25°C Klebsiella pneumoniae
0.118
-
NADP+ in 50 mM Tris-HCl buffer (pH 8.0), 20 mM MgCl2, at 25°C Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Escherichia coli P00350
-
-
Klebsiella pneumoniae Q762L5
-
-
Klebsiella pneumoniae NTUH-K2044 Q762L5
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA column chromatography Escherichia coli
Ni2+-NTA column chromatography Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NADP+
-
Escherichia coli D-ribulose 5-phosphate + CO2 + NADPH + H+
-
?
6-phospho-D-gluconate + NADP+
-
Klebsiella pneumoniae D-ribulose 5-phosphate + CO2 + NADPH + H+
-
?
6-phospho-D-gluconate + NADP+
-
Klebsiella pneumoniae NTUH-K2044 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Escherichia coli
homodimer x-ray crystallography Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
6-phosphogluconate dehydrogenase
-
Escherichia coli
6-phosphogluconate dehydrogenase
-
Klebsiella pneumoniae
6PGDH
-
Escherichia coli
6PGDH
-
Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Escherichia coli
NADP+
-
Klebsiella pneumoniae