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Literature summary for 1.1.1.432 extracted from

  • Sharma, M.; Lingford, J.P.; Petricevic, M.; Snow, A.J.D.; Zhang, Y.; Jaerva, M.A.; Mui, J.W.; Scott, N.E.; Saunders, E.C.; Mao, R.; Epa, R.; da Silva, B.M.; Pires, D.E.V.; Ascher, D.B.; McConville, M.J.; Davies, G.J.; Williams, S.J.; Goddard-Borger, E.D.
    Oxidative desulfurization pathway for complete catabolism of sulfoquinovose by bacteria (2022), Proc. Natl. Acad. Sci. USA, 119, e2116022119 .
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with NADPH and as ternary product complex by soaking crystals with D-glucose, to 1.5 A resolution. SmoB is an (alpha/beta)8 TIM barrel fold with a C-terminal cofactor binding site. Glucose interacts with residues Arg152 and Lys120 as well as His151 and Tyr76 within the conserved catalytic tetrad His/Tyr/Lys/Asp. SmoB exists as a compact trimer Agrobacterium fabrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + NADP+ Agrobacterium fabrum
-
 6-dehydro-D-glucose + NADPH + H+
-
 ?
D-glucose + NADP+ Agrobacterium fabrum ATCC 33970
-
 6-dehydro-D-glucose + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium fabrum A9CEY6
-
-
Agrobacterium fabrum ATCC 33970 A9CEY6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + NADP+
-
 Agrobacterium fabrum 6-dehydro-D-glucose + NADPH + H+
-
 ?
D-glucose + NADP+
-
 Agrobacterium fabrum ATCC 33970 6-dehydro-D-glucose + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
trimer crystallization data Agrobacterium fabrum

Synonyms

Synonyms Comment Organism
Atu3278
-
 Agrobacterium fabrum
smoB
-
 Agrobacterium fabrum

Cofactor

Cofactor Comment Organism Structure
additional information NAD+ does not support the reaction, no binding is observed for NADH Agrobacterium fabrum
NADP+
-
 Agrobacterium fabrum
NADPH binds to SmoB with Kd of about 2 microM Agrobacterium fabrum

General Information

General Information Comment Organism
evolution the sulfoquinovose monooxygenase pathway is distributed across Alpha- and Betaproteobacteria and is especially prevalent within the Rhizobiales order Agrobacterium fabrum
physiological function enzyme is part of a pathway for sulfoquinovose degradation. The NAD(P)H-dependent oxidoreductase reduces the 6-oxo-glucose to glucose, enabling entry into primary metabolic pathways Agrobacterium fabrum