Cloned (Comment) | Organism |
---|---|
gene xyl1, recombinant expression in Escherichia coli strain BL21(DE3), coexpression with glucose dehydrogenase (GDH) gene of Bacillus cereus strain ATCC 14579 | Yamadazyma tenuis |
gene xyr8, recombinant expression in Escherichia coli strain BL21(DE3), coexpression with glucose dehydrogenase (GDH) gene of Bacillus cereus strain ATCC 14579 | Candida tropicalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADPH + H+ | Yamadazyma tenuis | - |
xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | Candida tropicalis | - |
xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | Yamadazyma tenuis CBS 4435 | - |
xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | Candida tropicalis IFO 0618 | - |
xylitol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida tropicalis | P87039 | - |
- |
Candida tropicalis IFO 0618 | P87039 | - |
- |
Yamadazyma tenuis | O74237 | - |
- |
Yamadazyma tenuis CBS 4435 | O74237 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADPH + H+ | - |
Yamadazyma tenuis | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | - |
Candida tropicalis | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | - |
Yamadazyma tenuis CBS 4435 | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | - |
Candida tropicalis IFO 0618 | xylitol + NADP+ | - |
r | |
additional information | analysis and comparison of activities of differently expressed recombinant enzymes in xylitol production through whole-cell catalysis from pure xylose and glucose | Yamadazyma tenuis | ? | - |
- |
|
additional information | analysis and comparison of activities of differently expressed recombinant enzymes in xylitol production through whole-cell catalysis from pure xylose and glucose | Candida tropicalis | ? | - |
- |
|
additional information | analysis and comparison of activities of differently expressed recombinant enzymes in xylitol production through whole-cell catalysis from pure xylose and glucose | Yamadazyma tenuis CBS 4435 | ? | - |
- |
|
additional information | analysis and comparison of activities of differently expressed recombinant enzymes in xylitol production through whole-cell catalysis from pure xylose and glucose | Candida tropicalis IFO 0618 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
NAD(P)H-dependent D-xylose reductase | UniProt | Yamadazyma tenuis |
NADPH-dependent D-xylose reductase II,III | UniProt | Candida tropicalis |
Texr | - |
Yamadazyma tenuis |
TrxR | - |
Candida tropicalis |
XYL1 | - |
Yamadazyma tenuis |
xyr8 | - |
Candida tropicalis |
XyrB | - |
Candida tropicalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Yamadazyma tenuis |
25 | - |
assay at | Candida tropicalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Yamadazyma tenuis |
7 | - |
assay at | Candida tropicalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Yamadazyma tenuis | |
NADP+ | - |
Candida tropicalis | |
NADPH | - |
Yamadazyma tenuis | |
NADPH | - |
Candida tropicalis |
General Information | Comment | Organism |
---|---|---|
physiological function | xylitol production through whole-cell catalysis from pure xylose and glucose, and xylitol production from cornstalk hydrolysate | Yamadazyma tenuis |
physiological function | xylitol production through whole-cell catalysis from pure xylose and glucose, and xylitol production from cornstalk hydrolysate, method optimization, overview | Candida tropicalis |