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Literature summary for 1.1.1.431 extracted from

  • Chang, Z.; Liu, D.; Yang, Z.; Wu, J.; Zhuang, W.; Niu, H.; Ying, H.
    Efficient xylitol production from cornstalk hydrolysate using engineered Escherichia coli whole cells (2018), J. Agric. Food Chem., 66, 13209-13216 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene xyl1, recombinant expression in Escherichia coli strain BL21(DE3), coexpression with glucose dehydrogenase (GDH) gene of Bacillus cereus strain ATCC 14579 Yamadazyma tenuis
gene xyr8, recombinant expression in Escherichia coli strain BL21(DE3), coexpression with glucose dehydrogenase (GDH) gene of Bacillus cereus strain ATCC 14579 Candida tropicalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NADPH + H+ Yamadazyma tenuis
-
xylitol + NADP+
-
r
D-xylose + NADPH + H+ Candida tropicalis
-
xylitol + NADP+
-
r
D-xylose + NADPH + H+ Yamadazyma tenuis CBS 4435
-
xylitol + NADP+
-
r
D-xylose + NADPH + H+ Candida tropicalis IFO 0618
-
xylitol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Candida tropicalis P87039
-
-
Candida tropicalis IFO 0618 P87039
-
-
Yamadazyma tenuis O74237
-
-
Yamadazyma tenuis CBS 4435 O74237
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylose + NADPH + H+
-
Yamadazyma tenuis xylitol + NADP+
-
r
D-xylose + NADPH + H+
-
Candida tropicalis xylitol + NADP+
-
r
D-xylose + NADPH + H+
-
Yamadazyma tenuis CBS 4435 xylitol + NADP+
-
r
D-xylose + NADPH + H+
-
Candida tropicalis IFO 0618 xylitol + NADP+
-
r
additional information analysis and comparison of activities of differently expressed recombinant enzymes in xylitol production through whole-cell catalysis from pure xylose and glucose Yamadazyma tenuis ?
-
-
additional information analysis and comparison of activities of differently expressed recombinant enzymes in xylitol production through whole-cell catalysis from pure xylose and glucose Candida tropicalis ?
-
-
additional information analysis and comparison of activities of differently expressed recombinant enzymes in xylitol production through whole-cell catalysis from pure xylose and glucose Yamadazyma tenuis CBS 4435 ?
-
-
additional information analysis and comparison of activities of differently expressed recombinant enzymes in xylitol production through whole-cell catalysis from pure xylose and glucose Candida tropicalis IFO 0618 ?
-
-

Synonyms

Synonyms Comment Organism
NAD(P)H-dependent D-xylose reductase UniProt Yamadazyma tenuis
NADPH-dependent D-xylose reductase II,III UniProt Candida tropicalis
Texr
-
Yamadazyma tenuis
TrxR
-
Candida tropicalis
XYL1
-
Yamadazyma tenuis
xyr8
-
Candida tropicalis
XyrB
-
Candida tropicalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Yamadazyma tenuis
25
-
assay at Candida tropicalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Yamadazyma tenuis
7
-
assay at Candida tropicalis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Yamadazyma tenuis
NADP+
-
Candida tropicalis
NADPH
-
Yamadazyma tenuis
NADPH
-
Candida tropicalis

General Information

General Information Comment Organism
physiological function xylitol production through whole-cell catalysis from pure xylose and glucose, and xylitol production from cornstalk hydrolysate Yamadazyma tenuis
physiological function xylitol production through whole-cell catalysis from pure xylose and glucose, and xylitol production from cornstalk hydrolysate, method optimization, overview Candida tropicalis