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Literature summary for 1.1.1.431 extracted from
- Discovery and characterization of a xylose reductase from Zymomonas mobilis ZM4 (2011), Biotechnol. Lett., 33, 2127-2133.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ZMO0976, expression of His-tagged enzyme in Escherichia coli strain UT5600 | Zymomonas mobilis subsp. mobilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Zymomonas mobilis subsp. mobilis | |
| 1.8 | - |
benzaldehyde | pH 7.2, temperature not specified in the publication | Zymomonas mobilis subsp. mobilis |  |
| 4.2 | - |
furfural | pH 7.2, temperature not specified in the publication | Zymomonas mobilis subsp. mobilis | |
| 258 | - |
D-xylose | pH 7.2, temperature not specified in the publication | Zymomonas mobilis subsp. mobilis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylose + NADH + H+ | Zymomonas mobilis subsp. mobilis | - |
xylitol + NAD+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zymomonas mobilis subsp. mobilis | F2YCN5 | ssp. mobilis, and strain A3, an engineered strain ZM4 that is adapted to 5% D-xylose, gene ZMO0976 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain UT5600 by nickel affinity chromatography | Zymomonas mobilis subsp. mobilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-hydroxymethylfurfural + NADH + H+ | low activity | Zymomonas mobilis subsp. mobilis | (furan-2,5-diyl)dimethanol + NAD+ | - |
r | |
| acetaldehyde + NADH + H+ | - |
Zymomonas mobilis subsp. mobilis | ethanol + NAD+ | - |
r | |
| benzaldehyde + NADH + H+ | best substrate | Zymomonas mobilis subsp. mobilis | benzyl alcohol + NAD+ | - |
r | |
| D-xylose + NADH + H+ | - |
Zymomonas mobilis subsp. mobilis | xylitol + NAD+ | - |
r | |
| furfural + NADH + H+ | - |
Zymomonas mobilis subsp. mobilis | (furan-2-yl)methanol + NAD+ | - |
r | |
| xylulose + NADH + H+ | - |
Zymomonas mobilis subsp. mobilis | ? + NAD+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| xylose reductase | - |
Zymomonas mobilis subsp. mobilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Zymomonas mobilis subsp. mobilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Zymomonas mobilis subsp. mobilis | |
| NADH | - |
Zymomonas mobilis subsp. mobilis | |
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