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Literature summary for 1.1.1.431 extracted from

  • Yokoyama, S.-I.; Suzuki, T.; Kawai, K.; Horitsu, H.; Takamizawa, K.
    Purification, characterization and structure analysis of NADPH-dependent D-xylose reductases from Candida tropicalis (1995), J. Ferment. Bioeng., 79, 217-223.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
NADPH pH 7.0, D-xylose reductase 3 Candida tropicalis
0.014
-
NADPH pH 7.0, D-xylose reductase 1 Candida tropicalis
0.018
-
NADPH pH 7.0, D-xylose reductase 2 Candida tropicalis
30
-
D-xylose pH 7.0, D-xylose reductase 2 Candida tropicalis
34
-
D-xylose pH 7.0, D-xylose reductase 3 Candida tropicalis
37
-
D-xylose pH 7.0, D-xylose reductase 1 Candida tropicalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
D-xylose reductase 1, D-xylose reductase 2, D-xylose reductase 3, gel filtration Candida tropicalis
364978
-
2 * 364978, D-xylose reductase 1, ion-spray mass spectrometry Candida tropicalis
365540
-
2 * 365540, D-xylose reductase 2, ion-spray mass spectrometry Candida tropicalis

Organism

Organism UniProt Comment Textmining
Candida tropicalis
-
IF0 0618
-
Candida tropicalis IF0 0618
-
IF0 0618
-

Purification (Commentary)

Purification (Comment) Organism
-
Candida tropicalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
47.8
-
D-xylose reductase 3 Candida tropicalis
56.9
-
D-xylose reductase 1 Candida tropicalis
81
-
D-xylose reductase 2 Candida tropicalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + NADPH + H+ D-xylose reductase 1: 49% of the activity with D-xylose, D-xylose reductase 2: 40% of the activity with D-xylose, D-xylose reductase 3: 33% of the activity with D-xylose Candida tropicalis ?
-
?
D-galactose + NADPH + H+ D-xylose reductase 1: 49% of the activity with D-xylose, D-xylose reductase 2: 40% of the activity with D-xylose, D-xylose reductase 3: 33% of the activity with D-xylose Candida tropicalis IF0 0618 ?
-
?
D-glucose + NADPH + H+ D-xylose reductase 1: 10% of the activity with D-xylose, D-xylose reductase 2: 11% of the activity with D-xylose, D-xylose reductase 3: 11% of the activity with D-xylose Candida tropicalis ?
-
?
D-glucose + NADPH + H+ D-xylose reductase 1: 10% of the activity with D-xylose, D-xylose reductase 2: 11% of the activity with D-xylose, D-xylose reductase 3: 11% of the activity with D-xylose Candida tropicalis IF0 0618 ?
-
?
D-xylose + NADPH + H+
-
Candida tropicalis xylitol + NADP+
-
?
D-xylose + NADPH + H+
-
Candida tropicalis IF0 0618 xylitol + NADP+
-
?
DL-glyceraldehyde + NADPH + H+ D-xylose reductase 1: 200% of the activity with D-xylose, D-xylose reductase 2: 268% of the activity with D-xylose, D-xylose reductase 3: 143% of the activity with D-xylose Candida tropicalis glycerol + NADP+
-
?
DL-glyceraldehyde + NADPH + H+ D-xylose reductase 1: 200% of the activity with D-xylose, D-xylose reductase 2: 268% of the activity with D-xylose, D-xylose reductase 3: 143% of the activity with D-xylose Candida tropicalis IF0 0618 glycerol + NADP+
-
?
L-arabinose + NADPH + H+ D-xylose reductase 1: 117% of the activity with D-xylose, D-xylose reductase 2: 120% of the activity with D-xylose, D-xylose reductase 3: 101% of the activity with D-xylose Candida tropicalis L-arabinitol + NADP+
-
?
L-arabinose + NADPH + H+ D-xylose reductase 1: 117% of the activity with D-xylose, D-xylose reductase 2: 120% of the activity with D-xylose, D-xylose reductase 3: 101% of the activity with D-xylose Candida tropicalis IF0 0618 L-arabinitol + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 364978, D-xylose reductase 1, ion-spray mass spectrometry Candida tropicalis
dimer 2 * 365540, D-xylose reductase 2, ion-spray mass spectrometry Candida tropicalis

Synonyms

Synonyms Comment Organism
D-xylose reductase 1
-
Candida tropicalis
D-xylose reductase 2
-
Candida tropicalis
D-xylose reductase 3
-
Candida tropicalis
XR1
-
Candida tropicalis
XR2
-
Candida tropicalis
XR3
-
Candida tropicalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
1 h, D-xylose reductase 1, D-xylose reductase 2, complete loss of activity Candida tropicalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
D-xylose reductase 1, D-xylose reductase 2 Candida tropicalis

Cofactor

Cofactor Comment Organism Structure
NADPH no activity with NADH Candida tropicalis

pI Value

Organism Comment pI Value Maximum pI Value
Candida tropicalis D-xylose reductase 2, isoelectric focusing, pH-range: 2.5-5.0
-
4.1
Candida tropicalis D-xylose reductase 1, isoelectric focusing, pH-range: 2.5-5.0
-
4.15