KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
NADPH | pH 7.0, D-xylose reductase 3 | Candida tropicalis | |
0.014 | - |
NADPH | pH 7.0, D-xylose reductase 1 | Candida tropicalis | |
0.018 | - |
NADPH | pH 7.0, D-xylose reductase 2 | Candida tropicalis | |
30 | - |
D-xylose | pH 7.0, D-xylose reductase 2 | Candida tropicalis | |
34 | - |
D-xylose | pH 7.0, D-xylose reductase 3 | Candida tropicalis | |
37 | - |
D-xylose | pH 7.0, D-xylose reductase 1 | Candida tropicalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
D-xylose reductase 1, D-xylose reductase 2, D-xylose reductase 3, gel filtration | Candida tropicalis |
364978 | - |
2 * 364978, D-xylose reductase 1, ion-spray mass spectrometry | Candida tropicalis |
365540 | - |
2 * 365540, D-xylose reductase 2, ion-spray mass spectrometry | Candida tropicalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida tropicalis | - |
IF0 0618 | - |
Candida tropicalis IF0 0618 | - |
IF0 0618 | - |
Purification (Comment) | Organism |
---|---|
- |
Candida tropicalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47.8 | - |
D-xylose reductase 3 | Candida tropicalis |
56.9 | - |
D-xylose reductase 1 | Candida tropicalis |
81 | - |
D-xylose reductase 2 | Candida tropicalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + NADPH + H+ | D-xylose reductase 1: 49% of the activity with D-xylose, D-xylose reductase 2: 40% of the activity with D-xylose, D-xylose reductase 3: 33% of the activity with D-xylose | Candida tropicalis | ? | - |
? | |
D-galactose + NADPH + H+ | D-xylose reductase 1: 49% of the activity with D-xylose, D-xylose reductase 2: 40% of the activity with D-xylose, D-xylose reductase 3: 33% of the activity with D-xylose | Candida tropicalis IF0 0618 | ? | - |
? | |
D-glucose + NADPH + H+ | D-xylose reductase 1: 10% of the activity with D-xylose, D-xylose reductase 2: 11% of the activity with D-xylose, D-xylose reductase 3: 11% of the activity with D-xylose | Candida tropicalis | ? | - |
? | |
D-glucose + NADPH + H+ | D-xylose reductase 1: 10% of the activity with D-xylose, D-xylose reductase 2: 11% of the activity with D-xylose, D-xylose reductase 3: 11% of the activity with D-xylose | Candida tropicalis IF0 0618 | ? | - |
? | |
D-xylose + NADPH + H+ | - |
Candida tropicalis | xylitol + NADP+ | - |
? | |
D-xylose + NADPH + H+ | - |
Candida tropicalis IF0 0618 | xylitol + NADP+ | - |
? | |
DL-glyceraldehyde + NADPH + H+ | D-xylose reductase 1: 200% of the activity with D-xylose, D-xylose reductase 2: 268% of the activity with D-xylose, D-xylose reductase 3: 143% of the activity with D-xylose | Candida tropicalis | glycerol + NADP+ | - |
? | |
DL-glyceraldehyde + NADPH + H+ | D-xylose reductase 1: 200% of the activity with D-xylose, D-xylose reductase 2: 268% of the activity with D-xylose, D-xylose reductase 3: 143% of the activity with D-xylose | Candida tropicalis IF0 0618 | glycerol + NADP+ | - |
? | |
L-arabinose + NADPH + H+ | D-xylose reductase 1: 117% of the activity with D-xylose, D-xylose reductase 2: 120% of the activity with D-xylose, D-xylose reductase 3: 101% of the activity with D-xylose | Candida tropicalis | L-arabinitol + NADP+ | - |
? | |
L-arabinose + NADPH + H+ | D-xylose reductase 1: 117% of the activity with D-xylose, D-xylose reductase 2: 120% of the activity with D-xylose, D-xylose reductase 3: 101% of the activity with D-xylose | Candida tropicalis IF0 0618 | L-arabinitol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 364978, D-xylose reductase 1, ion-spray mass spectrometry | Candida tropicalis |
dimer | 2 * 365540, D-xylose reductase 2, ion-spray mass spectrometry | Candida tropicalis |
Synonyms | Comment | Organism |
---|---|---|
D-xylose reductase 1 | - |
Candida tropicalis |
D-xylose reductase 2 | - |
Candida tropicalis |
D-xylose reductase 3 | - |
Candida tropicalis |
XR1 | - |
Candida tropicalis |
XR2 | - |
Candida tropicalis |
XR3 | - |
Candida tropicalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, D-xylose reductase 1, D-xylose reductase 2, complete loss of activity | Candida tropicalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
D-xylose reductase 1, D-xylose reductase 2 | Candida tropicalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | no activity with NADH | Candida tropicalis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Candida tropicalis | D-xylose reductase 2, isoelectric focusing, pH-range: 2.5-5.0 | - |
4.1 |
Candida tropicalis | D-xylose reductase 1, isoelectric focusing, pH-range: 2.5-5.0 | - |
4.15 |