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Literature summary for 1.1.1.427 extracted from

  • Amako, K.; Fujita, K.; Iwamoto, C.; Sengee, M.; Fuchigami, K.; Fukumoto, J.; Ogishi, Y.; Kishimoto, R.; Goda, K.
    NADP(+)-dependent D-arabinose dehydrogenase shows a limited contribution to erythroascorbic acid biosynthesis and oxidative stress resistance in Saccharomyces cerevisiae (2006), Biosci. Biotechnol. Biochem., 70, 3004-3012 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
110
-
L-Xylose recombinant subunit ARA1p, at pH 8.2 and 25°C Saccharomyces cerevisiae
160
-
D-Lyxose recombinant subunit ARA1p, at pH 8.2 and 25°C Saccharomyces cerevisiae
210
-
L-gulose recombinant subunit ARA1p, at pH 8.2 and 25°C Saccharomyces cerevisiae
220
-
D-arabinose recombinant subunit ARA1p, at pH 8.2 and 25°C Saccharomyces cerevisiae
310
-
L-fucose recombinant subunit ARA1p, at pH 8.2 and 25°C Saccharomyces cerevisiae
330
-
L-galactose recombinant subunit ARA1p, at pH 8.2 and 25°C Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000 75000 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae YPH250
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinose + NADP+
-
Saccharomyces cerevisiae D-arabinono-1,4-1actone + NADPH + H+
-
?
D-arabinose + NADP+
-
Saccharomyces cerevisiae YPH250 D-arabinono-1,4-1actone + NADPH + H+
-
?
D-lyxose + NADP+
-
Saccharomyces cerevisiae ?
-
?
D-lyxose + NADP+
-
Saccharomyces cerevisiae YPH250 ?
-
?
L-fucose + NADP+
-
Saccharomyces cerevisiae ?
-
?
L-fucose + NADP+
-
Saccharomyces cerevisiae YPH250 ?
-
?
L-galactose + NADP+
-
Saccharomyces cerevisiae ?
-
?
L-galactose + NADP+
-
Saccharomyces cerevisiae YPH250 ?
-
?
L-gulose + NADP+
-
Saccharomyces cerevisiae ?
-
?
L-xylose + NADP+ preferred substrate Saccharomyces cerevisiae ?
-
?
additional information the recombinant subunit ARA1p shows 0.5% activity with NAD+ compared to NADPH and has no activity with glycerol Saccharomyces cerevisiae ?
-
-
additional information the recombinant subunit ARA1p shows 0.5% activity with NAD+ compared to NADPH and has no activity with glycerol Saccharomyces cerevisiae YPH250 ?
-
-

Subunits

Subunits Comment Organism
heterodimer 1 * 40000 + 1 * 39000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
ARA
-
Saccharomyces cerevisiae
Ara1p subunit Saccharomyces cerevisiae
NADP(+)-dependent D-arabinose dehydrogenase
-
Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADP+ preferred cofactor Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction an enzyme-deficient mutant shows increased susceptibility to H2O2 and diamide but not to menadione or tert-butylhydroperoxide Saccharomyces cerevisiae