Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
110 | - |
L-Xylose | recombinant subunit ARA1p, at pH 8.2 and 25°C | Saccharomyces cerevisiae | |
160 | - |
D-Lyxose | recombinant subunit ARA1p, at pH 8.2 and 25°C | Saccharomyces cerevisiae | |
210 | - |
L-gulose | recombinant subunit ARA1p, at pH 8.2 and 25°C | Saccharomyces cerevisiae | |
220 | - |
D-arabinose | recombinant subunit ARA1p, at pH 8.2 and 25°C | Saccharomyces cerevisiae | |
310 | - |
L-fucose | recombinant subunit ARA1p, at pH 8.2 and 25°C | Saccharomyces cerevisiae | |
330 | - |
L-galactose | recombinant subunit ARA1p, at pH 8.2 and 25°C | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | 75000 | gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae YPH250 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinose + NADP+ | - |
Saccharomyces cerevisiae | D-arabinono-1,4-1actone + NADPH + H+ | - |
? | |
D-arabinose + NADP+ | - |
Saccharomyces cerevisiae YPH250 | D-arabinono-1,4-1actone + NADPH + H+ | - |
? | |
D-lyxose + NADP+ | - |
Saccharomyces cerevisiae | ? | - |
? | |
D-lyxose + NADP+ | - |
Saccharomyces cerevisiae YPH250 | ? | - |
? | |
L-fucose + NADP+ | - |
Saccharomyces cerevisiae | ? | - |
? | |
L-fucose + NADP+ | - |
Saccharomyces cerevisiae YPH250 | ? | - |
? | |
L-galactose + NADP+ | - |
Saccharomyces cerevisiae | ? | - |
? | |
L-galactose + NADP+ | - |
Saccharomyces cerevisiae YPH250 | ? | - |
? | |
L-gulose + NADP+ | - |
Saccharomyces cerevisiae | ? | - |
? | |
L-xylose + NADP+ | preferred substrate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | the recombinant subunit ARA1p shows 0.5% activity with NAD+ compared to NADPH and has no activity with glycerol | Saccharomyces cerevisiae | ? | - |
- |
|
additional information | the recombinant subunit ARA1p shows 0.5% activity with NAD+ compared to NADPH and has no activity with glycerol | Saccharomyces cerevisiae YPH250 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 40000 + 1 * 39000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
ARA | - |
Saccharomyces cerevisiae |
Ara1p | subunit | Saccharomyces cerevisiae |
NADP(+)-dependent D-arabinose dehydrogenase | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | preferred cofactor | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | an enzyme-deficient mutant shows increased susceptibility to H2O2 and diamide but not to menadione or tert-butylhydroperoxide | Saccharomyces cerevisiae |