Literature summary for 1.1.1.427 extracted from

van Bergen, B.; Strasser, R.; Cyr, N.; Sheppard, J.D.; Jardim, A.
alpha,beta-Dicarbonyl reduction by Saccharomyces D-arabinose dehydrogenase (2006), Biochim. Biophys. Acta, 1760, 1636-1645 .

Search for more literature for 1.1.1.427

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli ER2566 cells	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.014	-	NADPH	at pH 7.0 and 25°C	Saccharomyces cerevisiae
4.2	-	2,3-Pentanedione	at pH 7.0 and 25°C	Saccharomyces cerevisiae
7.7	-	diacetyl	at pH 7.0 and 25°C	Saccharomyces cerevisiae
14.3	-	methylglyoxal	at pH 7.0 and 25°C	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Saccharomyces cerevisiae	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
75000	80000	gel filtration	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-
Saccharomyces cerevisiae BY4742	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,3-pentanedione + NADPH + H+	-	Saccharomyces cerevisiae	hydroxypentanone + NADP+	-	?
2,3-pentanedione + NADPH + H+	-	Saccharomyces cerevisiae BY4742	hydroxypentanone + NADP+	-	?
acetoin + NADP+	this back reaction only occurs at alkaline pH values (9.0-10.0)	Saccharomyces cerevisiae	diacetyl + NADPH + H+	-	r
acetoin + NADP+	this back reaction only occurs at alkaline pH values (9.0-10.0)	Saccharomyces cerevisiae BY4742	diacetyl + NADPH + H+	-	r
diacetyl + NADPH + H+	-	Saccharomyces cerevisiae	acetoin + NADP+	-	r
diacetyl + NADPH + H+	-	Saccharomyces cerevisiae BY4742	acetoin + NADP+	-	r
methylglyoxal + NADPH + H+	-	Saccharomyces cerevisiae	?	-	?
methylglyoxal + NADPH + H+	-	Saccharomyces cerevisiae BY4742	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 40000, SDS-PAGE	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
ARA1	-	Saccharomyces cerevisiae
Ara1p	-	Saccharomyces cerevisiae
D-arabinose dehydrogenase	-	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.7	-	NADPH	at pH 7.0 and 25°C	Saccharomyces cerevisiae
4.4	-	methylglyoxal	at pH 7.0 and 25°C	Saccharomyces cerevisiae
5.9	-	2,3-Pentanedione	at pH 7.0 and 25°C	Saccharomyces cerevisiae
6.9	-	diacetyl	at pH 7.0 and 25°C	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	5	pH optimum for the diacetyl reduction reaction	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	the enzyme specifically utilizes NADPH	Saccharomyces cerevisiae

General Information

General Information	Comment	Organism
malfunction	enzyme knockout mutants exhibit a decreased growth rate phenotype in media containing diacetyl	Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.3	-	methylglyoxal	at pH 7.0 and 25°C	Saccharomyces cerevisiae
0.9	-	diacetyl	at pH 7.0 and 25°C	Saccharomyces cerevisiae
1.41	-	2,3-Pentanedione	at pH 7.0 and 25°C	Saccharomyces cerevisiae
257	-	NADPH	at pH 7.0 and 25°C	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)