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Literature summary for 1.1.1.424 extracted from

  • Johnsen, U.; Dambeck, M.; Zaiss, H.; Fuhrer, T.; Soppa, J.; Sauer, U.; Schonheit, P.
    D-Xylose degradation pathway in the halophilic archaeon Haloferax volcanii (2009), J. Biol. Chem., 284, 27290-27303 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.424

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
 NADP+ pH 7.5, 42°C Haloferax volcanii
0.36
-
 NAD+ pH 7.5, 42°C Haloferax volcanii
4.7
-
 D-xylose pH 7.5, 42°C Haloferax volcanii
198
-
 D-glucose pH 7.5, 42°C Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
165000
-
 gel filtration Haloferax volcanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NAD+ Haloferax volcanii
-
 D-xylonolactone + NADH + H+
-
 ?
D-xylose + NAD+ Haloferax volcanii DSM 3757
-
 D-xylonolactone + NADH + H+
-
 ?
D-xylose + NADP+ Haloferax volcanii
-
 D-xylono-1,4-lactone + NADPH + H+
-
 ?
D-xylose + NADP+ Haloferax volcanii DSM 3757
-
 D-xylono-1,4-lactone + NADPH + H+
-
 ?
additional information Haloferax volcanii no substrates: L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose ?
-
-
additional information Haloferax volcanii DSM 3757 no substrates: L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose ?
-
-

Organism

Organism UniProt Comment Textmining
Haloferax volcanii D4GP29
-
-
Haloferax volcanii DSM 3757 D4GP29
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.8
-
 substrate D-glucose, pH 7.5, 42°C Haloferax volcanii
24.1
-
 substrate D-xylose, pH 7.5, 42°C Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + NADP+
-
 Haloferax volcanii D-gluconolactone + NADPH + H+
-
 ?
D-glucose + NADP+
-
 Haloferax volcanii DSM 3757 D-gluconolactone + NADPH + H+
-
 ?
D-xylose + NAD+
-
 Haloferax volcanii D-xylonolactone + NADH + H+
-
 ?
D-xylose + NAD+
-
 Haloferax volcanii DSM 3757 D-xylonolactone + NADH + H+
-
 ?
D-xylose + NADP+
-
 Haloferax volcanii D-xylono-1,4-lactone + NADPH + H+
-
 ?
D-xylose + NADP+
-
 Haloferax volcanii DSM 3757 D-xylono-1,4-lactone + NADPH + H+
-
 ?
additional information no substrates: L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose Haloferax volcanii ?
-
-
additional information no substrates: L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose Haloferax volcanii DSM 3757 ?
-
-

Subunits

Subunits Comment Organism
tetramer 4 * 55000, SDS-PAGE Haloferax volcanii

Synonyms

Synonyms Comment Organism
HVO_B0028
-
 Haloferax volcanii
xacA
-
 Haloferax volcanii

Cofactor

Cofactor Comment Organism Structure
NAD+ NADP+ is preferred over NAD+ Haloferax volcanii
NADP+ NADP+ is preferred over NAD+ Haloferax volcanii

General Information

General Information Comment Organism
physiological function D-xylose is oxidized exclusively to the tricarboxylic acid cycle intermediate alpha-ketoglutarate, involving D-xylose dehydrogenase (HVO_B0028), a xylonate dehydratase (HVO_B0038A), 2-keto-3-deoxyxylonate dehydratase (HVO_B0027), and alpha-ketoglutarate semialdehyde dehydrogenase (HVO_B0039). An HVO_B0028 gene deletion mutant cannot grow on xylose but grows unaffected on glucose as the wild-type Haloferax volcanii