Protein Variants | Comment | Organism |
---|---|---|
K413Q | the acetylation surrogate mutant of isoform IDH2 exhibits lower oxidative reaction rates than wild type. 2-Hydroxyglutarate production by the mutant is largely diminished at the enzymatic and cellular level | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
isocitrate | mutant enzyme K413Q, at pH 8.0 and 37°C | Homo sapiens | |
0.005 | - |
isocitrate | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens | |
0.0176 | - |
NADP+ | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens | |
0.0569 | - |
NADP+ | mutant enzyme K413Q, at pH 8.0 and 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 200 mM used in assay conditions | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + CO2 + NADPH + H+ | Homo sapiens | - |
isocitrate + NADP+ | - |
r | |
isocitrate + NADP+ | Homo sapiens | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P48735 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
acetylation | SIRT3 deacetylates isoform IDH2 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
Flag-tag affinity column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + CO2 + NADPH + H+ | - |
Homo sapiens | isocitrate + NADP+ | - |
r | |
isocitrate + NADP+ | - |
Homo sapiens | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
IDH2 | - |
Homo sapiens |
isocitrate dehydrogenase 2 | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
36.5 | - |
melting temperature | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.9 | - |
isocitrate | mutant enzyme K413Q, at pH 8.0 and 37°C | Homo sapiens | |
7.9 | - |
NADP+ | mutant enzyme K413Q, at pH 8.0 and 37°C | Homo sapiens | |
15.5 | - |
isocitrate | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens | |
23.5 | - |
NADP+ | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |