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Literature summary for 1.1.1.42 extracted from

  • Smolkova, K.; Spackova, J.; Gotvaldova, K.; Dvorak, A.; Krenkova, A.; Hubalek, M.; Holendova, B.; Vitek, L.; Jezek, P.
    SIRT3 and GCN5L regulation of NADP+- and NADPH-driven reactions of mitochondrial isocitrate dehydrogenase IDH2 (2020), Sci. Rep., 10, 8677 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
K413Q the acetylation surrogate mutant of isoform IDH2 exhibits lower oxidative reaction rates than wild type. 2-Hydroxyglutarate production by the mutant is largely diminished at the enzymatic and cellular level Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
isocitrate mutant enzyme K413Q, at pH 8.0 and 37°C Homo sapiens
0.005
-
isocitrate wild type enzyme, at pH 8.0 and 37°C Homo sapiens
0.0176
-
NADP+ wild type enzyme, at pH 8.0 and 37°C Homo sapiens
0.0569
-
NADP+ mutant enzyme K413Q, at pH 8.0 and 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 200 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxoglutarate + CO2 + NADPH + H+ Homo sapiens
-
isocitrate + NADP+
-
r
isocitrate + NADP+ Homo sapiens
-
2-oxoglutarate + CO2 + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens P48735
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
acetylation SIRT3 deacetylates isoform IDH2 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
Flag-tag affinity column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + CO2 + NADPH + H+
-
Homo sapiens isocitrate + NADP+
-
r
isocitrate + NADP+
-
Homo sapiens 2-oxoglutarate + CO2 + NADPH + H+
-
r

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
IDH2
-
Homo sapiens
isocitrate dehydrogenase 2
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
36.5
-
melting temperature Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.9
-
isocitrate mutant enzyme K413Q, at pH 8.0 and 37°C Homo sapiens
7.9
-
NADP+ mutant enzyme K413Q, at pH 8.0 and 37°C Homo sapiens
15.5
-
isocitrate wild type enzyme, at pH 8.0 and 37°C Homo sapiens
23.5
-
NADP+ wild type enzyme, at pH 8.0 and 37°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens
NADPH
-
Homo sapiens