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Literature summary for 1.1.1.42 extracted from

  • Cho, H.J.; Cho, H.Y.; Park, J.W.; Kwon, O.S.; Lee, H.S.; Huh, T.L.; Kang, B.S.
    NADP+-dependent cytosolic isocitrate dehydrogenase provides NADPH in the presence of cadmium due to the moderate chelating effect of glutathione (2018), J. Biol. Inorg. Chem., 23, 849-860 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.42

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M sodium citrate, pH 5.5, 16% (w/v) PEG 4000, 20% (v/v) isopropanol Mus musculus

Protein Variants

Protein Variants Comment Organism
C245S the activities of the mutant in the presence of Cd2+ are decreased as that of wild type enzyme Mus musculus
C379S the activity of the mutant in the absence of Cd2+ is similar to that of the wild type enzyme, but the decrease of activity in the presence of Cd2+ is much reduced Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ above 0.1 mM, the enzyme loses activity, and at 2 mM Cd2+ most of the activity has disappeared. Chelation of Cd2+ by dithiothreitol cannot recover the lost enzyme activity. Inactivation of the enzyme by Cd2+ is less effective when the enzyme is activated with Cd2+ than Mg2+, More than 50% of the activity of the enzyme activated with 0.05 mM Cd2+ remains in the presence of 1 mM GSH Mus musculus
Mn2+ the specific activity of purified enzyme is increased by Mn2+ in the micromolar range Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Mus musculus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ the enzyme activity is increased at a Cd2+ concentration below 0.1 mM Mus musculus
Mg2+ the maximum activity with Mg2+ is slightly lower than that with Cd2+ Mus musculus
additional information Ca2+ does not activate Mg2+ Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Mus musculus
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus O88844
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
 Mus musculus 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
IDH
-
 Mus musculus
NADP+-dependent isocitrate dehydrogenase
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Mus musculus