Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
R153C | the mutation dramatically reduces the catalytic efficiency of the enzyme for isocitrate oxidation, which drops to 1.5% of the wild type enzyme. The mutant acquires a neomorphic ability of producing 2-hydroxyglutarate from 2-oxoglutarate | Escherichia coli |
R153H | the mutation dramatically reduces the catalytic efficiency of the enzyme for isocitrate oxidation, which drops to 0.6% of the wild type enzyme. The mutant acquires a neomorphic ability of producing 2-hydroxyglutarate from 2-oxoglutarate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00071 | - |
NADPH | mutant enzyme R153H, at pH 7.5 and 25°C | Escherichia coli | |
0.00072 | - |
NADPH | mutant enzyme R153C, at pH 7.5 and 25°C | Escherichia coli | |
0.011 | - |
NADP+ | mutant enzyme R153H, at pH 7.5 and 25°C | Escherichia coli | |
0.0121 | - |
NADP+ | wild type enzyme, at pH 7.5 and 25°C | Escherichia coli | |
0.0176 | - |
NADP+ | mutant enzyme R153C, at pH 7.5 and 25°C | Escherichia coli | |
0.0217 | - |
isocitrate | mutant enzyme R153H, at pH 7.5 and 25°C | Escherichia coli | |
0.0242 | - |
isocitrate | wild type enzyme, at pH 7.5 and 25°C | Escherichia coli | |
0.0318 | - |
isocitrate | mutant enzyme R153C, at pH 7.5 and 25°C | Escherichia coli | |
2.2 | - |
2-oxoglutarate | mutant enzyme R153C, at pH 7.5 and 25°C | Escherichia coli | |
3.3 | - |
2-oxoglutarate | mutant enzyme R153H, at pH 7.5 and 25°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Escherichia coli | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 2 mM used in assay conditions | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Escherichia coli | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P08200 | - |
- |
Purification (Comment) | Organism |
---|---|
TALON metal affinity resin column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADPH + H+ | the reaction is catalyzed by mutant enzymes R153H and R153C | Escherichia coli | 2-hydroxyglutarate + NADP+ | - |
? | |
isocitrate + NADP+ | - |
Escherichia coli | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
IDH1 | - |
Escherichia coli |
NADP+-IDH | - |
Escherichia coli |
NADP+-isocitrate dehydrogenase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
isocitrate | mutant enzyme R153H, at pH 7.5 and 25°C | Escherichia coli | |
0.3 | - |
isocitrate | mutant enzyme R153C, at pH 7.5 and 25°C | Escherichia coli | |
1 | - |
2-oxoglutarate | mutant enzyme R153C, at pH 7.5 and 25°C | Escherichia coli | |
1 | - |
2-oxoglutarate | mutant enzyme R153H, at pH 7.5 and 25°C | Escherichia coli | |
51.8 | - |
isocitrate | wild type enzyme, at pH 7.5 and 25°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
2-oxoglutarate | mutant enzyme R153H, at pH 7.5 and 25°C | Escherichia coli | |
0.45 | - |
2-oxoglutarate | mutant enzyme R153C, at pH 7.5 and 25°C | Escherichia coli | |
8 | - |
isocitrate | mutant enzyme R153H, at pH 7.5 and 25°C | Escherichia coli | |
9 | - |
isocitrate | mutant enzyme R153C, at pH 7.5 and 25°C | Escherichia coli | |
2100 | - |
isocitrate | wild type enzyme, at pH 7.5 and 25°C | Escherichia coli |