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Literature summary for 1.1.1.42 extracted from

  • Song, P.; Wang, M.; Zheng, Q.; Wang, P.; Zhu, G.
    Isocitrate dehydrogenase 1 from Acinetobacter baummanii (AbIDH1) enzymatic characterization and its regulation by phosphorylation (2021), Biochimie, 181, 77-85 .
    View publication on PubMed
Search for more literature for 1.1.1.42

Cloned(Commentary)

Cloned (Comment) Organism
expressipon in Escherichia coli Acinetobacter baumannii

Protein Variants

Protein Variants Comment Organism
S113A mutant enzyme with remarkably reduced enzymatic activity Acinetobacter baumannii
S113A the mutation remarkably reduces the enzymatic activity Acinetobacter baumannii
S113D the mutant lacks almost all enzymatic activity Acinetobacter baumannii
S113D the phosphorylation-mimicking mutant lacks almost all enzymatic activity Acinetobacter baumannii
S113E the mutant lacks almost all enzymatic activity Acinetobacter baumannii
S113E the phosphorylation-mimicking mutant lacks almost all enzymatic activity Acinetobacter baumannii
S113G mutant enzyme with remarkably reduced enzymatic activity Acinetobacter baumannii
S113G the mutation remarkably reduces the enzymatic activity Acinetobacter baumannii
S113T mutant enzyme with remarkably reduced enzymatic activity Acinetobacter baumannii
S113T the mutation remarkably reduces the enzymatic activity Acinetobacter baumannii
S113Y mutant enzyme with remarkably reduced enzymatic activity Acinetobacter baumannii
S113Y the mutation remarkably reduces the enzymatic activity Acinetobacter baumannii

General Stability

General Stability Organism
the activity of the enzyme treated with Escherichia coli isocitrate dehydrogenase kinase/phosphatase immediately decreases by 80% within 9 min Acinetobacter baumannii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0181
-
 NADP+ in the presence of Mg2+, pH and temperature not specified in the publication Acinetobacter baumannii
0.0181
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme Acinetobacter baumannii
0.0223
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H Acinetobacter baumannii
0.0261
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A Acinetobacter baumannii
0.0289
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E Acinetobacter baumannii
0.0466
-
 NADP+ in the presence of Mn2+, pH and temperature not specified in the publication Acinetobacter baumannii
0.0466
-
 NADP+ pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme Acinetobacter baumannii
0.0505
-
 isocitrate in the presence of Mn2+, pH and temperature not specified in the publication Acinetobacter baumannii
0.0505
-
 isocitrate pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme Acinetobacter baumannii
0.0654
-
 isocitrate in the presence of Mg2+, pH and temperature not specified in the publication Acinetobacter baumannii
0.0654
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme Acinetobacter baumannii
0.0879
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T Acinetobacter baumannii
0.1043
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A Acinetobacter baumannii
0.1169
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H Acinetobacter baumannii
0.1221
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A Acinetobacter baumannii
0.1414
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E Acinetobacter baumannii
0.2447
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T Acinetobacter baumannii
0.3276
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A Acinetobacter baumannii
0.8546
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G Acinetobacter baumannii
2.525
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G Acinetobacter baumannii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Acinetobacter baumannii
Mn2+ activates Acinetobacter baumannii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83500
-
 gel filtration Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Acinetobacter baumannii
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
isocitrate + NADP+ Acinetobacter baumannii overall reaction 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter baumannii
-
-
-
Acinetobacter baumannii V5VCI0
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the enzyme is phosphorylated at Ser113 by Escherichia coli isocitrate dehydrogenase kinase/phosphatase Acinetobacter baumannii

Purification (Commentary)

Purification (Comment) Organism
-
 Acinetobacter baumannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
 Acinetobacter baumannii 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
isocitrate + NADP+ overall reaction Acinetobacter baumannii 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
additional information the enzyme is a substrate for Escherichia coli isocitrate dehydrogenase kinase/phosphatase Acinetobacter baumannii ?
-
-

Subunits

Subunits Comment Organism
homodimer 2 * 45000, SDS-PAGE Acinetobacter baumannii
homodimer 2 * 83500, SDS-PAGE Acinetobacter baumannii

Synonyms

Synonyms Comment Organism
AbIDH1
-
 Acinetobacter baumannii
IDH1
-
 Acinetobacter baumannii
isocitrate dehydrogenase 1
-
 Acinetobacter baumannii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A Acinetobacter baumannii
2.1
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A Acinetobacter baumannii
2.9
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T Acinetobacter baumannii
3 6 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E Acinetobacter baumannii
3.2
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T Acinetobacter baumannii
3.4
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G Acinetobacter baumannii
4
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G Acinetobacter baumannii
28.5
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A Acinetobacter baumannii
29.2
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H Acinetobacter baumannii
29.8
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme Acinetobacter baumannii
30.3
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E Acinetobacter baumannii
31.3
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A Acinetobacter baumannii
33.4
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme Acinetobacter baumannii
37
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H Acinetobacter baumannii
50.4
-
 isocitrate pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme Acinetobacter baumannii
60.1
-
 NADP+ pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme Acinetobacter baumannii

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Acinetobacter baumannii
NADP+ NADP+-dependent enzyme Acinetobacter baumannii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E Acinetobacter baumannii
1.1
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A Acinetobacter baumannii
1.3
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H Acinetobacter baumannii
2
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G Acinetobacter baumannii
4
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113G Acinetobacter baumannii
6
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A Acinetobacter baumannii
13
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T Acinetobacter baumannii
16
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113A Acinetobacter baumannii
33
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S113T Acinetobacter baumannii
250
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22E Acinetobacter baumannii
300
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22A Acinetobacter baumannii
320
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, mutant enzyme S22H Acinetobacter baumannii
500
-
 isocitrate pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme Acinetobacter baumannii
1000
-
 isocitrate pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme Acinetobacter baumannii
1300
-
 NADP+ pH 8.0, 25°C, in presence of Mn2+, wild-type enzyme Acinetobacter baumannii
1700
-
 NADP+ pH 8.0, 25°C, in presence of Mg2+, wild-type enzyme Acinetobacter baumannii