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Literature summary for 1.1.1.42 extracted from
- Decrease in the cytosolic NADP+-dependent isocitrate dehydrogenase activity through porcine sperm capacitation (2018), Biochem. Biophys. Res. Commun., 497, 374-380 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH5alpha cells | Sus scrofa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Sus scrofa | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 1 mM used in assay conditions | Sus scrofa |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | Sus scrofa | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | A0A4X1UZU9 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | tyrosine phosphorylated enzyme shows a significantly lowered enzymatic activity | Sus scrofa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione-Sepharose column chromatography and DEAE-Sepharose chromatography | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| semen | mainly localized to the principal piece of the porcine sperm flagellum | Sus scrofa | - |
| testis | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | - |
Sus scrofa | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Sus scrofa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IDPc | - |
Sus scrofa |
| NADP+-dependent isocitrate dehydrogenase | - |
Sus scrofa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Sus scrofa | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Sus scrofa | enzyme activities decrease following capacitation of sperm | down |
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Release 2023.1 (January 2023)
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