Cloned (Comment) | Organism |
---|---|
gene Tc00.1047053506925.319 or IDH2, recombinant expression of the N-terminally His6-tagged enzyme in Escherichia coli | Trypanosoma cruzi |
gene Tc00.1047053511575.60 or IDH1, recombinant expression of the N-terminally His6-tagged enzyme in Escherichia coli, co-expression with bacterial GroEL/GroES chaperones is required for IDH1 in order to achieve a soluble enzyme | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, overview | Trypanosoma cruzi | |
0.021 | - |
DL-isocitrate | pH and temperature not specified in the publication | Trypanosoma cruzi | |
0.045 | - |
NADP+ | pH and temperature not specified in the publication | Trypanosoma cruzi | |
0.054 | - |
DL-isocitrate | pH and temperature not specified in the publication | Trypanosoma cruzi | |
0.056 | - |
NADP+ | pH and temperature not specified in the publication | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | IDH2 contains a C-terminal PTS1 signal, but this enzyme is mostly localized in the cytosol, expression levels of the cytosolic isoform are significantly increased in amastigotes, metacyclic trypomastigotes and cell-derived trypomastigotes compared to epimastigotes | Trypanosoma cruzi | 5829 | - |
glycosome | IDH2 contains a C-terminal PTS1 signal, but this enzyme is mostly localized in the cytosol of epimastigotes | Trypanosoma cruzi | 20015 | - |
mitochondrion | mitochondrial targeting peptide signal is predicted for IDH1 | Trypanosoma cruzi | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Trypanosoma cruzi | |
Mn2+ | activates, preferred divalent cation | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, recombinant N-terminally His6-tagged enzyme, SDS-PAGE | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NADP+ | Trypanosoma cruzi | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q4DG65 | gene Tc00.1047053511575.60 or IDH1; gene idh, isozyme IDH1 | - |
Trypanosoma cruzi | Q4E4L7 | gene Tc00.1047053506925.319 or IDH2; gene idh, two isozymes | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | - |
Trypanosoma cruzi | - |
epimastigote | - |
Trypanosoma cruzi | - |
additional information | expression levels of the cytosolic isoform are significantly increased in amastigotes, metacyclic trypomastigotes and cell-derived trypomastigotes compared to epimastigotes | Trypanosoma cruzi | - |
trypomastigote | - |
Trypanosoma cruzi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
67 | - |
purified recombinant N-terminally His6-tagged enzyme, pH and temperature not specified in the publication | Trypanosoma cruzi |
219 | - |
purified recombinant N-terminally His6-tagged enzyme, pH and temperature not specified in the publication | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NADP+ | - |
Trypanosoma cruzi | 2-oxoglutarate + CO2 + NADPH + H+ | - |
ir | |
DL-isocitrate + NADP+ | carboxylation of 2-oxoglutarate to produce isocitrate | Trypanosoma cruzi | 2-oxoglutarate + CO2 + NADPH + H+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, recombinant N-terminally His6-tagged enzyme, SDS-PAGE | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
IDH1 | - |
Trypanosoma cruzi |
NADP-dependent isocitrate dehydrogenase | - |
Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is completely inactive with NAD+ | Trypanosoma cruzi | |
NADP+ | - |
Trypanosoma cruzi |