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Literature summary for 1.1.1.42 extracted from

  • Popova, T.; Carvalho, M.A.; Matasova, L.; Medvedeva, L.
    Regulation of mitochondrial NADP-isocitrate dehydrogenase in rat heart during ischemia (2006), Mol. Cell. Biochem., 294, 97-105.
    View publication on PubMed
Search for more literature for 1.1.1.42

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate
-
 Rattus norvegicus
citrate
-
 Rattus norvegicus
Fe2+ the inhibitory effect of the Fe2+ and H2O2 mixture associated with the generation of hydroxyl radicals is lower in enzyme from ischemic heart compared to enzyme from normoxic heart Rattus norvegicus
glutathione
-
 Rattus norvegicus
H2O2 the inhibitory effect of the Fe2+ and H2O2 mixture associated with the generation of hydroxyl radicals is lower in enzyme from ischemic heart compared to enzyme from normoxic heart Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 DL-isocitrate enzyme from ischemic heart, pH 7.8, 25°C Rattus norvegicus
0.017
-
 isocitrate pH 7.8, 25°C, enzyme from ischemic heart Rattus norvegicus
0.045
-
 DL-isocitrate enzyme from normoxic heart, pH 7.8, 25°C Rattus norvegicus
0.045
-
 isocitrate pH 7.8, 25°C, enzyme from normoxic heart Rattus norvegicus
0.046
-
 NADP+ enzyme from ischemic heart, pH 7.8, 25°C Rattus norvegicus
0.046
-
 NADP+ enzyme from normoxic heart, pH 7.8, 25°C Rattus norvegicus
0.046
-
 NADP+ pH 7.8, 25°C, enzyme from ischemic heart Rattus norvegicus
0.046
-
 NADP+ pH 7.8, 25°C, enzyme from normoxic heart Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-
cytosol increase in enzyme activity during ischemia Rattus norvegicus 5829
-
mitochondrion
-
 Rattus norvegicus 5739
-
mitochondrion increase in enzyme activity during ischemia, enzyme from ischemic heart mitochondria demonstrates higher activation energy and lower thermal stability and differs in KM-value and regulation Rattus norvegicus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ KM-value for enzyme from normoxic heart, 0.42 mM, for enzyme from ischemic heart 0.15 mM Rattus norvegicus
Mn2+ Km-value: 0.42 mM for enzyme from normoxic heart, 0.15 mM for enzyme from ischemic heart Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Rattus norvegicus P56574 albino
-

Purification (Commentary)

Purification (Comment) Organism
NADP-isocitrate dehydrogenase from the normoxic and ischemic rat myocardium Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
heart regulation of enzyme during heart ischemia. Ischemia results in increase in enzyme activity, enzyme from ischemic heart mitochondria demonstrates higher activation energy and lower thermal stability and differs in KM-value and regulation Rattus norvegicus
-
heart increase of enzyme activity in the cytosol and mitochondria of ischemic heart Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3 8
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-isocitrate + NADP+
-
 Rattus norvegicus 2-oxoglutarate + CO2 + NADPH
-
 ?
isocitrate + NADP+
-
 Rattus norvegicus 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
NADP-ICDH
-
 Rattus norvegicus
NADP-isocitrate dehydrogenase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 enzyme from ischemic heart Rattus norvegicus
50
-
 enzyme from normoxic heart Rattus norvegicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about 50% of maximal activity, 50°C: about 70% of maximal activity, enzyme from ischemic heart Rattus norvegicus
35 70 35°C: about 45% of maximal activity, 70°C: about 60% of maximal activity, enzyme from normoxic heart Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05
-
 Fe2+ enzyme from ischemic heart, pH 7.8, 25°C Rattus norvegicus
0.05
-
 Fe2+ enzyme from normoxic heart, pH 7.8, 25°C Rattus norvegicus
0.05
-
 Fe2+ pH 7.8, 25°C, enzyme from normoxic heart Rattus norvegicus
0.05
-
 Fe2+ pH 7.8, 25°C,enzyme from ischemic heart Rattus norvegicus
0.08
-
 2-oxoglutarate enzyme from normoxic heart, pH 7.8, 25°C Rattus norvegicus
0.08
-
 2-oxoglutarate pH 7.8, 25°C,enzyme from normoxic heart Rattus norvegicus
0.15
-
 glutathione enzyme from normoxic heart, pH 7.8, 25°C Rattus norvegicus
0.15
-
 glutathione pH 7.8, 25°C, enzyme from normoxic heart Rattus norvegicus
0.159
-
 citrate enzyme from normoxic heart, pH 7.8, 25°C Rattus norvegicus
0.159
-
 citrate pH 7.8, 25°C, enzyme from normoxic heart Rattus norvegicus
0.25
-
 2-oxoglutarate enzyme from ischemic heart, pH 7.8, 25°C Rattus norvegicus
0.25
-
 2-oxoglutarate pH 7.8, 25°C,enzyme from ischemic heart Rattus norvegicus
0.28
-
 citrate enzyme from ischemic heart, pH 7.8, 25°C Rattus norvegicus
0.28
-
 citrate pH 7.8, 25°C,enzyme from ischemic heart Rattus norvegicus
0.45
-
 H2O2 enzyme from normoxic heart, pH 7.8, 25°C Rattus norvegicus
0.45
-
 H2O2 pH 7.8, 25°C, enzyme from normoxic heart Rattus norvegicus
1.63
-
 H2O2 enzyme from ischemic heart, pH 7.8, 25°C Rattus norvegicus
1.63
-
 H2O2 pH 7.8, 25°C,enzyme from ischemic heart Rattus norvegicus