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Literature summary for 1.1.1.42 extracted from

  • Lebedeva, N.V.; Malinina, N.V.; Ivanovsky, R.N.
    A comparative study of the isocitrate dehydrogenases of Chlorobium limicola forma thiosulfatophilum and Rhodopseudomonas palustris (2002), Microbiology, 71, 657-662.
No PubMed abstract available
Search for more literature for 1.1.1.42

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate 50% inhibition of the forward reaction at 1.5 mM, 99% inhibition at 15 mM Chlorobium limicola
2-oxoglutarate 50% inhibition of the forward reaction at 1.5 mM, 95% inhibition at 15 mM Rhodopseudomonas palustris
isocitrate 50% inhibition of the reverse reaction at 0.1 mM, 97% inhibition at 1.2 mM Chlorobium limicola
isocitrate 70% inhibition of the reverse reaction at 0.1 mM, 99% inhibition at 1.2 mM Rhodopseudomonas palustris
additional information no inhibition by glyoxylate Chlorobium limicola
additional information no inhibition by glyoxylate Rhodopseudomonas palustris
NADP+ 37% inhibition of the reverse reaction at 4.0 mM Chlorobium limicola
NADP+ 35% inhibition of the reverse reaction at 4.0 mM Rhodopseudomonas palustris
NADPH 42% inhibition of the forward reaction at 0.5 mM Chlorobium limicola
NADPH 66% inhibition of the forward reaction at 0.5 mM Rhodopseudomonas palustris
NaHCO3 28% inhibition of the forward reaction at 10 mM Chlorobium limicola
NaHCO3 9% inhibition of the forward reaction at 10 mM Rhodopseudomonas palustris
oxaloacetate 18.6% inhibition at 2.5 mM Chlorobium limicola
oxaloacetate 15.5% inhibition at 2.5 mM Rhodopseudomonas palustris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 isocitrate pH 8.0, forward reaction Chlorobium limicola
0.007
-
 NADP+ pH 8.0, forward reaction Chlorobium limicola
0.009
-
 isocitrate pH 8.0, forward reaction Rhodopseudomonas palustris
0.01
-
 NADPH pH 6.5, reverse reaction Chlorobium limicola
0.017
-
 NADP+ pH 8.0, forward reaction Rhodopseudomonas palustris
0.034
-
 NADPH pH 6.5, reverse reaction Rhodopseudomonas palustris
0.304
-
 2-oxoglutarate pH 6.5, reverse reaction Chlorobium limicola
0.398
-
 2-oxoglutarate pH 6.5, reverse reaction Rhodopseudomonas palustris
11.68
-
 CO2 pH 6.5, reverse reaction Chlorobium limicola
13.82
-
 CO2 pH 6.5, reverse reaction Rhodopseudomonas palustris

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Chlorobium limicola 5829
-
cytosol
-
 Rhodopseudomonas palustris 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation is required, optimal activity at 2 mM in the forward reaction, and at 200 mM in the reverse reaction Rhodopseudomonas palustris
Mg2+ divalent cation is required, optimal activity at 40 mM in the forward reaction, and at 250 mM in the reverse reaction Chlorobium limicola
Mn2+ divalent cation is required, optimal activity at 1 mM in the forward reaction, and at 20 mM in the reverse reaction Rhodopseudomonas palustris
Mn2+ divalent cation is required, optimal activity at 10 mM in the forward reaction, and at 50 mM in the reverse reaction Chlorobium limicola

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64400
-
 gel filtration Chlorobium limicola
69100
-
 gel filtration Rhodopseudomonas palustris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Chlorobium limicola metabolic regulation, involved in the tricarboxylic acid cycle 2-oxoglutarate + CO2 + NADPH + H+
-
 r
isocitrate + NADP+ Rhodopseudomonas palustris metabolic regulation, involved in the tricarboxylic acid cycle 2-oxoglutarate + CO2 + NADPH + H+
-
 r
isocitrate + NADP+ Rhodopseudomonas palustris 285 metabolic regulation, involved in the tricarboxylic acid cycle 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Chlorobium limicola
-
 formerly Chlorobium thiosulfatophilum, strain L
-
Rhodopseudomonas palustris
-
-
-
Rhodopseudomonas palustris 285
-
-
-

Purification (Commentary)

Purification (Comment) Organism
41fold by ultracentrifugation, ammonium sulfate fractionation, gel filtration and ion exchange chromatography, and desalting Chlorobium limicola
by ultracentrifugation, ammonium sulfate fractionation, gel filtration and ion exchange chromatography, and desalting Rhodopseudomonas palustris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.04
-
 purified enzyme, reverse reaction Chlorobium limicola
0.28
-
 purified enzyme, forward reaction Chlorobium limicola

Storage Stability

Storage Stability Organism
-18°C, purified enzyme, 50% glycerol, stable for at least 1.5-2 months Chlorobium limicola
-18°C, purified enzyme, 50% glycerol, stable for at least 1.5-2 months Rhodopseudomonas palustris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + CO2 + NADPH
-
 Chlorobium limicola isocitrate + NADP+
-
 r
2-oxoglutarate + CO2 + NADPH
-
 Rhodopseudomonas palustris isocitrate + NADP+
-
 r
2-oxoglutarate + CO2 + NADPH
-
 Rhodopseudomonas palustris 285 isocitrate + NADP+
-
 r
isocitrate + NADP+
-
 Chlorobium limicola 2-oxoglutarate + CO2 + NADPH + H+
-
 r
isocitrate + NADP+
-
 Rhodopseudomonas palustris 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
isocitrate + NADP+ metabolic regulation, involved in the tricarboxylic acid cycle Chlorobium limicola 2-oxoglutarate + CO2 + NADPH + H+
-
 r
isocitrate + NADP+ metabolic regulation, involved in the tricarboxylic acid cycle Rhodopseudomonas palustris 2-oxoglutarate + CO2 + NADPH + H+
-
 r
isocitrate + NADP+
-
 Rhodopseudomonas palustris 285 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
isocitrate + NADP+ metabolic regulation, involved in the tricarboxylic acid cycle Rhodopseudomonas palustris 285 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
ICDH
-
 Chlorobium limicola
ICDH
-
 Rhodopseudomonas palustris
isocitrate dehydrogenase
-
 Chlorobium limicola
isocitrate dehydrogenase
-
 Rhodopseudomonas palustris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 5 min, complete loss of activity Chlorobium limicola
50
-
 5 min, complete loss of activity Rhodopseudomonas palustris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1
-
 reverse reaction Rhodopseudomonas palustris
6.7
-
 reverse reaction Chlorobium limicola
8.1
-
 forward reaction Chlorobium limicola
8.3
-
 forward reaction Rhodopseudomonas palustris

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Chlorobium limicola
NADP+
-
 Rhodopseudomonas palustris
NADPH
-
 Chlorobium limicola
NADPH
-
 Rhodopseudomonas palustris

pI Value

Organism Comment pI Value Maximum pI Value
Rhodopseudomonas palustris isoelectric focusing
-
 4.9
Chlorobium limicola isoelectric focusing
-
 6