Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain erg26 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
D39V | inactive | Arabidopsis thaliana |
D70A | inactive | Arabidopsis thaliana |
D70V | inactive | Arabidopsis thaliana |
K163I | inactive | Arabidopsis thaliana |
R318I | the mutant shows slightly reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
R326I | inactive | Arabidopsis thaliana |
S131A | the mutant shows reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
S133A | the mutant shows reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
S133T | the mutant shows reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
S133Y | inactive | Arabidopsis thaliana |
T129V | inactive | Arabidopsis thaliana |
Y159F | inactive | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
4alpha-carboxy-cholest-7-en-3beta-ol | mutant enzyme S133A, pH and temperature not specified in the publication | Arabidopsis thaliana | |
0.13 | - |
4alpha-carboxy-cholest-7-en-3beta-ol | wild type enzyme, pH and temperature not specified in the publication | Arabidopsis thaliana | |
0.2 | - |
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol | mutant enzyme S133A, pH and temperature not specified in the publication | Arabidopsis thaliana | |
0.26 | - |
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol | wild type enzyme, pH and temperature not specified in the publication | Arabidopsis thaliana | |
0.51 | - |
4alpha-carboxy-cholest-7-en-3beta-ol | mutant enzyme S133T, pH and temperature not specified in the publication | Arabidopsis thaliana | |
0.77 | - |
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol | mutant enzyme S133T, pH and temperature not specified in the publication | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3beta-hydroxy,4alpha-carboxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-sterol + NAD+ | - |
Arabidopsis thaliana | 4alpha,14-dimethyl-5alpha,9beta-9,19-cycloergost-24(28)-en-3beta-ol + CO2 + NADH + H+ | - |
? | |
4alpha-carboxy-4beta,14alpha-dimethyl-9beta,19-cyclo-5alpha-cholest-24-en-3beta-ol + NAD+ | - |
Arabidopsis thaliana | cholest-7-en-3-one + CO2 + NADH + H+ | - |
? | |
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol + NAD+ | - |
Arabidopsis thaliana | ? + CO2 + NADH + H+ | - |
? | |
4alpha-carboxy-cholest-7-en-3beta-ol + NAD+ | - |
Arabidopsis thaliana | ? + CO2 + NADH + H+ | - |
? | |
4alpha-methyl-cholest-8,24-dien-3-one + NAD+ | - |
Arabidopsis thaliana | ? + CO2 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
3beta-hydroxysteroid-dehydrogenase/C4-decarboxylase | - |
Arabidopsis thaliana |
3betaHSD/D | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strong preference for NAD+ | Arabidopsis thaliana |