Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | the enzyme can be activated by low concentrations of ATP. At high concentrations ATP is competitive with NAD+ for binding to the catalytic site | Homo sapiens | |
citrate | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) CodonPlus cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
alphagamma heterodimer with the active site bound with either Mg2+ alone or Mg2+ and ATP, and the allosteric site bound with Mg2+, citrate and ATP, hanging drop vapor diffusion method, using 0.2 M sodium citrate, pH 8.0, and 20% (w/v) PEG3350 or 0.2 M sodium citrate, pH 5.5, and 24% (v/v) PEG400 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Homo sapiens | |
Mn2+ | 2 mM used in assay conditions | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | Homo sapiens | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P50213 and O43837 and P51553 | subunits alpha, beta and gamma | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | - |
Homo sapiens | 2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 1 * 39000 + 1 * 39000 + 2 * 37000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
IDH3 | - |
Homo sapiens |
NAD-dependent isocitrate dehydrogenase | - |
Homo sapiens |
NAD-IDH | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |