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Literature summary for 1.1.1.41 extracted from

  • Sun, P.; Bai, T.; Ma, T.; Ding, J.
    Molecular mechanism of the dual regulatory roles of ATP on the alphagamma heterodimer of human NAD-dependent isocitrate dehydrogenase (2020), Sci. Rep., 10, 6225 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ATP the enzyme can be activated by low concentrations of ATP. At high concentrations ATP is competitive with NAD+ for binding to the catalytic site Homo sapiens
citrate
-
Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) CodonPlus cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
alphagamma heterodimer with the active site bound with either Mg2+ alone or Mg2+ and ATP, and the allosteric site bound with Mg2+, citrate and ATP, hanging drop vapor diffusion method, using 0.2 M sodium citrate, pH 8.0, and 20% (w/v) PEG3350 or 0.2 M sodium citrate, pH 5.5, and 24% (v/v) PEG400 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
NADH
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Homo sapiens
Mn2+ 2 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NAD+ Homo sapiens
-
2-oxoglutarate + CO2 + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P50213 and O43837 and P51553 subunits alpha, beta and gamma
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NAD+
-
Homo sapiens 2-oxoglutarate + CO2 + NADH + H+
-
?

Subunits

Subunits Comment Organism
heterotetramer 1 * 39000 + 1 * 39000 + 2 * 37000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
IDH3
-
Homo sapiens
NAD-dependent isocitrate dehydrogenase
-
Homo sapiens
NAD-IDH
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens