Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21star(DE3) cells | Trypanosoma brucei brucei |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 100 mM HEPES-NaOH buffer, pH 7.2, 200 mM NaCl and 22% (w/v) PEG6000 | Trypanosoma brucei brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Trypanosoma brucei brucei | 5777 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 0.5 mM used in assay conditions | Trypanosoma brucei brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97500 | - |
gel filtration | Trypanosoma brucei brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | Trypanosoma brucei brucei | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | Trypanosoma brucei brucei ILtat 1.4 | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei brucei | - |
- |
- |
Trypanosoma brucei brucei ILtat 1.4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography, and Superdex 200 gel filtration | Trypanosoma brucei brucei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.7 | - |
with NADP+ as cosubstrate, at pH 8.0, temperature not specified in the publication | Trypanosoma brucei brucei |
22.1 | - |
with NAD+ as cosubstrate, at pH 8.0, temperature not specified in the publication | Trypanosoma brucei brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | - |
Trypanosoma brucei brucei | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | the enzyme has considerably high activity with NAD+ compared with NADP+ as electron acceptor | Trypanosoma brucei brucei | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | - |
Trypanosoma brucei brucei ILtat 1.4 | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | the enzyme has considerably high activity with NAD+ compared with NADP+ as electron acceptor | Trypanosoma brucei brucei ILtat 1.4 | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NADP+ | the enzyme has considerably high activity with NAD+ compared with NADP+ as electron acceptor | Trypanosoma brucei brucei | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | the enzyme has considerably high activity with NAD+ compared with NADP+ as electron acceptor | Trypanosoma brucei brucei ILtat 1.4 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 60000, His6-SUMO-tagged enzyme, SDS-PAGE | Trypanosoma brucei brucei |
Synonyms | Comment | Organism |
---|---|---|
IDH1 | - |
Trypanosoma brucei brucei |
NAD+-dependent type 1 IDH | - |
Trypanosoma brucei brucei |
type 1 isocitrate dehydrogenase | - |
Trypanosoma brucei brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Trypanosoma brucei brucei |