Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Methylobacillus flagellatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | complete inhibition at 2 mM (pH 6.0) | Methylobacillus flagellatus | |
Cu2+ | complete inhibition at 2 mM (pH 6.0) | Methylobacillus flagellatus | |
additional information | the enzyme is unaffected by the addition of 5 mM L-glutamate, L-glutamine, 2-oxoglutarate, oxaloacetate, cis-aconitate, citrate, pyruvate, malate, fumarate, succinate, CoA, AcCoA, NADH, and NADPH or 2 mM ADP and AMP | Methylobacillus flagellatus | |
Zn2+ | NAD+-linked activity at pH 8.5 is strongly inhibited by Zn2+ but partially restored at pH 6.0 | Methylobacillus flagellatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
isocitrate | at pH 8.5 and 42°C | Methylobacillus flagellatus | |
0.113 | - |
NAD+ | at pH 8.5 and 42°C | Methylobacillus flagellatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ can act as a significant substitute by providing up to 58-75% of the enzyme's maximal activity | Methylobacillus flagellatus | |
Mn2+ | Mn2+ enhances the activity the most effectively | Methylobacillus flagellatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83600 | - |
gel filtration | Methylobacillus flagellatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | Methylobacillus flagellatus | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
r | |
isocitrate + NAD+ | Methylobacillus flagellatus ATCC51484 | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylobacillus flagellatus | - |
- |
- |
Methylobacillus flagellatus ATCC51484 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity resin column chromatography | Methylobacillus flagellatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + APAD+ | 55% activity compared to NAD+ | Methylobacillus flagellatus | 2-oxoglutarate + CO2 + APADH | - |
r | |
isocitrate + APAD+ | 55% activity compared to NAD+ | Methylobacillus flagellatus ATCC51484 | 2-oxoglutarate + CO2 + APADH | - |
r | |
isocitrate + NAD+ | - |
Methylobacillus flagellatus | 2-oxoglutarate + CO2 + NADH + H+ | - |
r | |
isocitrate + NAD+ | 100% activity | Methylobacillus flagellatus | 2-oxoglutarate + CO2 + NADH + H+ | - |
r | |
isocitrate + NAD+ | - |
Methylobacillus flagellatus ATCC51484 | 2-oxoglutarate + CO2 + NADH + H+ | - |
r | |
isocitrate + NAD+ | 100% activity | Methylobacillus flagellatus ATCC51484 | 2-oxoglutarate + CO2 + NADH + H+ | - |
r | |
isocitrate + NHD+ | 4.0% activity compared to NAD+ | Methylobacillus flagellatus | 2-oxoglutarate + CO2 + NHDH | - |
r | |
isocitrate + NHD+ | 4.0% activity compared to NAD+ | Methylobacillus flagellatus ATCC51484 | 2-oxoglutarate + CO2 + NHDH | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45000, SDS-PAGE | Methylobacillus flagellatus |
homodimer | 2 * 44600, calculated from amino acid sequence | Methylobacillus flagellatus |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Methylobacillus flagellatus |
NAD+-specific IDH | - |
Methylobacillus flagellatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Methylobacillus flagellatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | the enzyme retains full activity at 50°C and loses ca. 50% of its activity after 1 h of incubation at 75°C | Methylobacillus flagellatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
166 | - |
NAD+ | at pH 8.5 and 42°C | Methylobacillus flagellatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Methylobacillus flagellatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the catalytic efficiency for NAD+ is 5times higher for NAD+ than for NADP+ | Methylobacillus flagellatus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Methylobacillus flagellatus | isoelectric focusing | - |
5.5 |
Methylobacillus flagellatus | calculated from amino acid sequence | - |
5.9 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1500 | - |
NAD+ | at pH 8.5 and 42°C | Methylobacillus flagellatus |