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Literature summary for 1.1.1.41 extracted from

  • Sun, P.; Ma, T.; Zhang, T.; Zhu, H.; Zhang, J.; Liu, Y.; Ding, J.
    Molecular basis for the function of the alphabeta heterodimer of human NAD-dependent isocitrate dehydrogenase (2019), J. Biol. Chem., 294, 16214-16227 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ADP
-
Homo sapiens
citrate
-
Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Codon-Plus cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
alphabeta heterodimer with the alpha subunit in apo form and in Ca2_-bound, NAD-bound, and NADH-bound forms, hanging drop vapor diffusion method, using either containing 8% (v/v) tacsimate (pH 6.0) and 20% (w/v) PEG 3350 or 0.2 M calcium acetate (pH 7.5) and 20% (w/v) PEG 3350 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
NADH NADH can compete with NAD+ to bind to the active site and inhibits the activity of the alphabeta heterodimer Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Homo sapiens
Mn2+ 2 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NAD+ Homo sapiens
-
2-oxoglutarate + CO2 + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P50213 and O43837 subunits alpha and beta
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NAD+
-
Homo sapiens 2-oxoglutarate + CO2 + NADH + H+
-
?

Subunits

Subunits Comment Organism
heterotetramer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
NAD-dependent isocitrate dehydrogenase
-
Homo sapiens
NAD-IDH
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens