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Literature summary for 1.1.1.41 extracted from

  • Sun, P.; Liu, Y.; Ma, T.; Ding, J.
    Structure and allosteric regulation of human NAD-dependent isocitrate dehydrogenase (2020), Cell Discov., 6, 94 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ADP
-
Homo sapiens
citrate
-
Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Codon-Plus cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
isoform IDH3 containing a beta mutant in apo form, hanging drop vapor diffusion method, using 0.05M NH4Cl, 0.05 Bis-Tris (pH 6.5), and 30% (w/v) pentaerythritol ethoxylate Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 2 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NAD+ Homo sapiens
-
2-oxoglutarate + CO2 + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P50213 and O43837 and P51553 subunits alpha, beta and gamma
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NAD+
-
Homo sapiens 2-oxoglutarate + CO2 + NADH + H+
-
?

Subunits

Subunits Comment Organism
heterooctamer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
IDH3
-
Homo sapiens
NAD-dependent isocitrate dehydrogenase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens