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Literature summary for 1.1.1.41 extracted from

  • Steen, I.H.; Madern, D.; Karlström, M.; Lien, T.; Ladenstein, R.; Birkeland, N.K.
    Comparison of isocitrate dehydrogenase from three hyperthermophiles reveals differences in thermostability, cofactor specificity, oligomeric state, and phylogenetic affiliation (2001), J. Biol. Chem., 276, 43924-43931.
    View publication on PubMed
Search for more literature for 1.1.1.41

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0683
-
 NAD+ pH 8.0, 70°C, the Km-value for NADP+ is about 65fold higher than the KM-value for NAD+ Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44300
-
 2 * 44300, calculated from sequence Pyrococcus furiosus
70000
-
 analytical ultracentrifugation Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U488
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NAD+ low activity with NADP+. Km-value for NADP+ is about 65fold higher than the KM-value for NAD+ Pyrococcus furiosus 2-oxoglutarate + CO2 + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 44300, calculated from sequence Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
NAD-IDH
-
 Pyrococcus furiosus
PF0202
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
 above Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 thermal denaturation is an irreversible process Pyrococcus furiosus
104
-
 the Tm-value is 103.7°C Pyrococcus furiosus