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Literature summary for 1.1.1.41 extracted from
- Identification of cofactor discrimination sites in NAD-isocitrate dehydrogenase from Pyrococcus furiosus (2002), Arch. Microbiol., 178, 297-300.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D328K | the mutant shows dual coenzyme specificity | Pyrococcus furiosus |
| D328K/I329Y | introduction of the double mutation shifts the cofactor preference from NAD+ to NADP+ | Pyrococcus furiosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.066 | - |
NAD+ | pH 8.0, 70°C, mutant enzyme D328K | Pyrococcus furiosus |  |
| 0.068 | - |
NAD+ | pH 8.0, 70°C, wild-type enzyme | Pyrococcus furiosus | |
| 0.162 | - |
NADP+ | pH 8.0, 70°C, mutant enzyme D328K | Pyrococcus furiosus | |
| 0.211 | - |
NADP+ | pH 8.0, 70°C, mutant enzyme D328K/I329Y | Pyrococcus furiosus | |
| 0.336 | - |
NAD+ | pH 8.0, 70°C, mutant enzyme D328K/I329Y | Pyrococcus furiosus | |
| 4.44 | - |
NADP+ | pH 8.0, 70°C, wild-type enzyme | Pyrococcus furiosus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | Q8U488 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NAD+ | wild-type enzyme is specific for NAD+, low activity with NADP+ as cofactor, mutant D328K shows dual coenzyme specificity, cofactor preference of mutant D328K/I329Y is shifted from NAD+ to NADP+ | Pyrococcus furiosus | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
| isocitrate + NADP+ | wild-type enzyme is specific for NAD+, low activity with NADP+ as cofactor, mutant D328K shows dual coenzyme specificity, cofactor preference of mutant D328K/I329Y is shifted from NAD+ to NADP+ | Pyrococcus furiosus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAD-IDH | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
assay at | Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 95 | activity at 80°C is about 50% compared to the activity at 90°C | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 102 | - |
the apparent melting temperature is 102.3°C, mutant enzyme D328K | Pyrococcus furiosus |
| 103 | - |
the apparent melting temperature is 102.9°C, mutant enzyme D328K/I329Y | Pyrococcus furiosus |
| 104 | - |
the apparent melting temperature is 103.7°C, wild-type enzyme | Pyrococcus furiosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 60.4 | - |
NAD+ | pH 8.0, 70°C, wild-type enzyme | Pyrococcus furiosus | |
| 93 | - |
NADP+ | pH 8.0, 70°C, wild-type enzyme | Pyrococcus furiosus | |
| 103.4 | - |
NAD+ | pH 8.0, 70°C, mutant enzyme D328K | Pyrococcus furiosus | |
| 105.6 | - |
NAD+ | pH 8.0, 70°C, mutant enzyme D328K/I329Y | Pyrococcus furiosus | |
| 133 | - |
NADP+ | pH 8.0, 70°C, mutant enzyme D328K | Pyrococcus furiosus | |
| 168 | - |
NADP+ | pH 8.0, 70°C, mutant enzyme D328K/I329Y | Pyrococcus furiosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pyrococcus furiosus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | wild-type enzyme is specific for NAD+, low activity with NADP+ as cofactor, mutant D328K shows dual coenzyme specificity, cofactor preference of mutant D328K/I329Y is shifted from NAD+ to NADP+ | Pyrococcus furiosus | |
| NADP+ | wild-type enzyme is specific for NAD+, low activity with NADP+ as cofactor, mutant D328K shows dual coenzyme specificity, cofactor preference of mutant D328K/I329Y is shifted from NAD+ to NADP+ | Pyrococcus furiosus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20.9 | - |
NADP+ | pH 8.0, 70°C, wild-type enzyme | Pyrococcus furiosus | |
| 314 | - |
NAD+ | pH 8.0, 70°C, mutant enzyme D328K/I329Y | Pyrococcus furiosus | |
| 796 | - |
NADP+ | pH 8.0, 70°C, mutant enzyme D328K/I329Y | Pyrococcus furiosus | |
| 821 | - |
NADP+ | pH 8.0, 70°C, mutant enzyme D328K | Pyrococcus furiosus | |
| 888 | - |
NAD+ | pH 8.0, 70°C, wild-type enzyme | Pyrococcus furiosus | |
| 1567 | - |
NAD+ | pH 8.0, 70°C, mutant enzyme D328K | Pyrococcus furiosus | |
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