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Literature summary for 1.1.1.406 extracted from

  • Schneider, K.H.; Jäkel, G.; Hoffmann, R.; Giffhorn, F.
    Enzyme evolution in Rhodobacter sphaeroides: Selection of a mutant expressing a new galactitol dehydrogenase and biochemical characterization of the enzyme (1995), Microbiology, 141, 1865-1873.
    View publication on PubMed

General Stability

General Stability Organism
sucrose stabilizes enzyme preparation Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl 13% inhibition at 1 mM, restored by addition of 2 mM MgCl2 Cereibacter sphaeroides
EDTA 68% inhibition at 1 mM, restored by addition of 2 mM MgCl2 Cereibacter sphaeroides
FeSO4
-
Cereibacter sphaeroides
ZnSO4
-
Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
NADH
-
Cereibacter sphaeroides
0.012
-
NAD+
-
Cereibacter sphaeroides
0.2
-
1,2-hexanediol
-
Cereibacter sphaeroides
48
-
dihydroxyacetone
-
Cereibacter sphaeroides
62
-
acetoin
-
Cereibacter sphaeroides
85
-
D-threitol
-
Cereibacter sphaeroides
144
-
L-erythrulose
-
Cereibacter sphaeroides
240
-
galactitol
-
Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 21% activation at 1 mM Cereibacter sphaeroides
Mg2+ 1 mM required for optimal activity Cereibacter sphaeroides
Mn2+ 1 mM required for optimal activity Cereibacter sphaeroides
Ni2+ 25% activation at 1 mM Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
4 * 28000, SDS-PAGE Cereibacter sphaeroides
110000
-
sucrose density gradient centrifugation Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-arabitol + NAD+ Cereibacter sphaeroides
-
? + NADH
-
?
D-ribulose + NADH Cereibacter sphaeroides
-
? + NAD+
-
?
D-threitol + NAD+ Cereibacter sphaeroides best substrate tested ? + NADH
-
?
D-xylulose + NADH Cereibacter sphaeroides
-
? + NAD+
-
?
galactitol + NAD+ Cereibacter sphaeroides
-
L-tagatose + NADH
-
r
L-erythrulose + NADH Cereibacter sphaeroides
-
? + NAD+
-
?
L-glucitol + NAD+ Cereibacter sphaeroides
-
? + NADH
-
?
xylitol + NAD+ Cereibacter sphaeroides high activity L-xylulose + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Cereibacter sphaeroides

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Cereibacter sphaeroides
-

Storage Stability

Storage Stability Organism
4°C, 20 mM Bis-Tris, pH 6.5, 1 mM MgCl2, 30% sucrose, stable for 3 months Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R)-butanol + NAD+
-
Cereibacter sphaeroides 2-butanone + NADH
-
?
(2S)-butanol + NAD+
-
Cereibacter sphaeroides 2-butanone + NADH
-
?
(2S,3S)-butanediol + NAD+
-
Cereibacter sphaeroides ? + NADH
-
?
1,2-butanediol + NAD+
-
Cereibacter sphaeroides ? + NADH
-
?
1,2-hexanediol + NAD+
-
Cereibacter sphaeroides ? + NADH
-
?
1,2-pentanediol + NAD+
-
Cereibacter sphaeroides ? + NADH
-
?
1,2-propanediol + NAD+
-
Cereibacter sphaeroides ? + NADH
-
?
2-hexanol + NAD+
-
Cereibacter sphaeroides 2-hexanone + NADH
-
?
2-propanol + NAD+
-
Cereibacter sphaeroides acetone + NADH
-
?
acetoin + NADH
-
Cereibacter sphaeroides ? + NAD+
-
?
D-arabitol + NAD+
-
Cereibacter sphaeroides ? + NADH
-
?
D-ribulose + NADH
-
Cereibacter sphaeroides ? + NAD+
-
?
D-threitol + NAD+ best substrate tested Cereibacter sphaeroides ? + NADH
-
?
D-xylulose + NADH
-
Cereibacter sphaeroides ? + NAD+
-
?
dihydroxyacetone + NADH
-
Cereibacter sphaeroides ? + NAD+
-
?
galactitol + NAD+
-
Cereibacter sphaeroides L-tagatose + NADH
-
r
glycerol + NAD+
-
Cereibacter sphaeroides DL-glyceraldehyde + NADH
-
r
L-erythrulose + NADH
-
Cereibacter sphaeroides ? + NAD+
-
?
L-glucitol + NAD+
-
Cereibacter sphaeroides ? + NADH
-
?
xylitol + NAD+ high activity Cereibacter sphaeroides L-xylulose + NADH + H+
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 28000, SDS-PAGE Cereibacter sphaeroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
50% loss of activity after 8 days Cereibacter sphaeroides
50
-
50% loss of activity after 12 min Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
reduction of acetoin Cereibacter sphaeroides
10.5
-
1,2-hexanediol oxidation Cereibacter sphaeroides

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
63% of the reduction activity Cereibacter sphaeroides
9
-
81% of the oxidation activity Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
NAD+ strictly specific Cereibacter sphaeroides
NADH strictly specific Cereibacter sphaeroides