BRENDA - Enzyme Database
show all sequences of 1.1.1.404

Biochemical characterization of the tetrachlorobenzoquinone reductase involved in the biodegradation of pentachlorophenol

Chen, L.; Yang, J.; Int. J. Mol. Sci. 9, 198-212 (2008)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
tetrachlorohydroquinone
activity of PcpD is stimulated by tetrachlorohydroquinone at low concentrations but inhibited at high concentrations. 83% increase in activity at 0.1 mM tetrachlorohydroquinone, 60% loss of activity at 0.2 mM tetrachlorohydroquinone
Sphingobium chlorophenolicum
Cloned(Commentary)
Commentary
Organism
expression in Eshcerichia coli
Sphingobium chlorophenolicum
Crystallization (Commentary)
Crystallization
Organism
homology modeling of structure. The active site is slightly positively charged and situated in a deep pit on the surface. The putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster. It is formed mainly by residues Ser78, Arg79, Phe225, Gly226, Ala227, Ala228, Leu229, Gln275 and the cofactor FMN
Sphingobium chlorophenolicum
Inhibitors
Inhibitors
Commentary
Organism
Structure
Pentachlorophenol
competitive, 55% loss of activity at 0.1 mM
Sphingobium chlorophenolicum
tetrachlorohydroquinone
activity of PcpD is stimulated by tetrachlorohydroquinone at low concentrations but inhibited at high concentrations. 83% increase in activity at 0.1 mM tetrachlorohydroquinone, 60% loss of activity at 0.2 mM tetrachlorohydroquinone
Sphingobium chlorophenolicum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
protein contains 1.7 mol of iron per mol
Sphingobium chlorophenolicum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
protein exists as trimer, dynamic light scattering
Sphingobium chlorophenolicum
36000
-
-
Sphingobium chlorophenolicum
36500
-
-
Sphingobium chlorophenolicum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sphingobium chlorophenolicum
Q47914
-
-
Sphingobium chlorophenolicum ATCC 39723
Q47914
-
-
Purification (Commentary)
Commentary
Organism
recombinant protein,. Purified enzyme is colorless
Sphingobium chlorophenolicum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.1
-
pH 7.0, 25░C
Sphingobium chlorophenolicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3,5,6-tetrachlorohydroquinone + NAD+
-
740552
Sphingobium chlorophenolicum
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+
-
-
-
?
2,3,5,6-tetrachlorohydroquinone + NAD+
-
740552
Sphingobium chlorophenolicum ATCC 39723
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotrimer
3 * 36000, SDS-PAGE, 3 * 36500, mass spectrometry, 3 * 36500, calculated
Sphingobium chlorophenolicum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Sphingobium chlorophenolicum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
-
70% of maximum activity
Sphingobium chlorophenolicum
8
-
20% of maximum activity
Sphingobium chlorophenolicum
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
the putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster
Sphingobium chlorophenolicum
[2Fe-2S]-center
the putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster
Sphingobium chlorophenolicum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
tetrachlorohydroquinone
activity of PcpD is stimulated by tetrachlorohydroquinone at low concentrations but inhibited at high concentrations. 83% increase in activity at 0.1 mM tetrachlorohydroquinone, 60% loss of activity at 0.2 mM tetrachlorohydroquinone
Sphingobium chlorophenolicum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Eshcerichia coli
Sphingobium chlorophenolicum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
the putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster
Sphingobium chlorophenolicum
[2Fe-2S]-center
the putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster
Sphingobium chlorophenolicum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
homology modeling of structure. The active site is slightly positively charged and situated in a deep pit on the surface. The putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster. It is formed mainly by residues Ser78, Arg79, Phe225, Gly226, Ala227, Ala228, Leu229, Gln275 and the cofactor FMN
Sphingobium chlorophenolicum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Pentachlorophenol
competitive, 55% loss of activity at 0.1 mM
Sphingobium chlorophenolicum
tetrachlorohydroquinone
activity of PcpD is stimulated by tetrachlorohydroquinone at low concentrations but inhibited at high concentrations. 83% increase in activity at 0.1 mM tetrachlorohydroquinone, 60% loss of activity at 0.2 mM tetrachlorohydroquinone
Sphingobium chlorophenolicum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
protein contains 1.7 mol of iron per mol
Sphingobium chlorophenolicum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
protein exists as trimer, dynamic light scattering
Sphingobium chlorophenolicum
36000
-
-
Sphingobium chlorophenolicum
36500
-
-
Sphingobium chlorophenolicum
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein,. Purified enzyme is colorless
Sphingobium chlorophenolicum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.1
-
pH 7.0, 25░C
Sphingobium chlorophenolicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3,5,6-tetrachlorohydroquinone + NAD+
-
740552
Sphingobium chlorophenolicum
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+
-
-
-
?
2,3,5,6-tetrachlorohydroquinone + NAD+
-
740552
Sphingobium chlorophenolicum ATCC 39723
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotrimer
3 * 36000, SDS-PAGE, 3 * 36500, mass spectrometry, 3 * 36500, calculated
Sphingobium chlorophenolicum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Sphingobium chlorophenolicum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
-
70% of maximum activity
Sphingobium chlorophenolicum
8
-
20% of maximum activity
Sphingobium chlorophenolicum
Other publictions for EC 1.1.1.404
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740063
Rudolph
A radical intermediate in the ...
Sphingobium chlorophenolicum
Biochemistry
53
6539-6549
2014
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1
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3
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1
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2
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2
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1
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1
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1
1
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744327
Rudolph
A radical intermediate in the ...
Sphingobium chlorophenolicum
Biochemistry
53
6539-6549
2014
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1
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3
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2
1
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2
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2
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1
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2
1
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1
1
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741332
Yadid
Sequestration of a highly reac ...
Sphingobium chlorophenolicum, Sphingobium chlorophenolicum RA2
Proc. Natl. Acad. Sci. USA
110
E2182-E2190
2013
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4
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2
2
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5
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1
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7
1
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4
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3
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5
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4
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2
2
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1
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7
1
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4
-
-
-
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1
1
-
5
5
740552
Chen
Biochemical characterization o ...
Sphingobium chlorophenolicum, Sphingobium chlorophenolicum ATCC 39723
Int. J. Mol. Sci.
9
198-212
2008
1
-
1
1
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2
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1
3
-
-
4
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1
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1
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2
1
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1
2
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2
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1
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1
2
1
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2
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1
3
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1
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1
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2
1
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1
2
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659022
Dai
A previously unrecognized step ...
Sphingobium chlorophenolicum, Sphingobium chlorophenolicum ATCC 39723
J. Bacteriol.
185
302-310
2003
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