Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.2 M potassium fluoride and 30% (w/v) PEG 3350 | Sorghum bicolor |
sitting drop vapor diffusion method, using 0.1 M potassium chloride, 0.1 M sodium citrate (pH 5.5) and 34% (w/v) pentaerythritol propoxylate | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
A339E | the mutant of isoform nonC4-NADP-ME shows increased catalytic efficiency compared to the wild type enzyme | Zea mays |
DelN | the mutant of isoform nonC4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme and is not inhibited by (S)-malate | Zea mays |
DelN/A339E | the mutant of isoform nonC4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme | Zea mays |
DelN/I140F/A339E | the mutant of isoform nonC4-NADP-ME shows increased catalytic efficiency compared to the wild type enzyme and is not inhibited by (S)-malate | Zea mays |
E339A | the mutant of isoform C4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme and is not inhibited by (S)-malate | Zea mays |
F140I | the mutant of isoform C4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme | Zea mays |
I140F | the mutant of isoform nonC4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme and is not inhibited by (S)-malate | Zea mays |
I140F/A339E | the mutant of isoform nonC4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme and is not inhibited by (S)-malate | Zea mays |
L544F | the mutant of isoform C4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme | Zea mays |
Q503E | the mutant of isoform C4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-malate | - |
Sorghum bicolor | |
(S)-malate | - |
Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0082 | - |
NADP+ | mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.0128 | - |
NADP+ | mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.0131 | - |
NADP+ | mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.0149 | - |
NADP+ | wild type isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.015 | - |
NADP+ | mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.0159 | - |
NADP+ | mutant E339A of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.0159 | - |
NADP+ | mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.023 | - |
NADP+ | mutant DelN of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.023 | - |
NADP+ | wild type isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.038 | - |
NADP+ | mutant I140F of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.0412 | - |
NADP+ | mutant I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.0503 | - |
NADP+ | mutant DelN/I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.23 | - |
(S)-malate | wild type isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.27 | - |
(S)-malate | mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.4 | - |
(S)-malate | mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.43 | - |
(S)-malate | wild type isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.46 | - |
(S)-malate | mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.47 | - |
(S)-malate | mutant E339A of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.47 | - |
(S)-malate | mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.58 | - |
(S)-malate | mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.85 | - |
(S)-malate | mutant I140F of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
0.95 | - |
(S)-malate | mutant DelN of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
1.05 | - |
(S)-malate | mutant DelN/I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
1.12 | - |
(S)-malate | mutant I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Zea mays | 9507 | - |
chloroplast | - |
Sorghum bicolor | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Zea mays | - |
pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | Sorghum bicolor | - |
pyruvate + CO2 + NADPH + H+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | Zea mays | - |
(S)-malate + NADP+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | Sorghum bicolor | - |
(S)-malate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | - |
cultivar v.3.1.1 | - |
Zea mays | - |
cultivar Ensembl-18 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Zea mays |
Ni-NTA agarose column chromatography | Sorghum bicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Zea mays | pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | - |
Sorghum bicolor | pyruvate + CO2 + NADPH + H+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | - |
Zea mays | (S)-malate + NADP+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | - |
Sorghum bicolor | (S)-malate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 66000, SDS-PAGE | Zea mays |
homotetramer | 4 * 66000, SDS-PAGE | Sorghum bicolor |
Synonyms | Comment | Organism |
---|---|---|
C4-NADP-ME | isoform | Zea mays |
C4-NADP-ME | isoform | Sorghum bicolor |
NADP-malic enzyme | - |
Zea mays |
NADP-malic enzyme | - |
Sorghum bicolor |
nicotinamide adenine dinucleotide phosphate-dependent malic enzyme | - |
Zea mays |
nicotinamide adenine dinucleotide phosphate-dependent malic enzyme | - |
Sorghum bicolor |
nonC4-NADP-ME | isoform | Zea mays |
nonC4-NADP-ME | isoform | Sorghum bicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
(S)-malate | mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
4 | - |
(S)-malate | mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
22 | - |
(S)-malate | mutant DelN of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
26.3 | - |
(S)-malate | wild type isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
27.4 | - |
(S)-malate | mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
28.1 | - |
(S)-malate | wild type isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
28.7 | - |
(S)-malate | mutant E339A of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
29.8 | - |
(S)-malate | mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
32.1 | - |
(S)-malate | mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
36.7 | - |
(S)-malate | mutant I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
39.6 | - |
(S)-malate | mutant I140F of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
85.8 | - |
(S)-malate | mutant DelN/I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Zea mays |
7.5 | 8 | - |
Sorghum bicolor |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
9.1 | - |
(S)-malate | wild type isoform C4-NADP-ME, at pH 8.0 and 25°C | Zea mays | |
13.3 | - |
(S)-malate | mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
13.4 | - |
(S)-malate | mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
15.8 | - |
(S)-malate | mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
16.7 | - |
(S)-malate | mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
19.4 | - |
(S)-malate | mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.7 | - |
(S)-malate | mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
10 | - |
(S)-malate | mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
23.2 | - |
(S)-malate | mutant DelN of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
32.8 | - |
(S)-malate | mutant I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
46.6 | - |
(S)-malate | mutant I140F of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
47.2 | - |
(S)-malate | mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
61.1 | - |
(S)-malate | mutant E339A of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
62.6 | - |
(S)-malate | wild type isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
64.8 | - |
(S)-malate | mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
81.7 | - |
(S)-malate | mutant DelN/I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
118.9 | - |
(S)-malate | mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C | Zea mays | |
122.2 | - |
(S)-malate | wild type isoform C4-NADP-ME, at pH 7.0 and 25°C | Zea mays |