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Literature summary for 1.1.1.40 extracted from

  • Alvarez, C.E.; Bovdilova, A.; Hoeppner, A.; Wolff, C.C.; Saigo, M.; Trajtenberg, F.; Zhang, T.; Buschiazzo, A.; Nagel-Steger, L.; Drincovich, M.F.; Lercher, M.J.; Maurino, V.G.
    Molecular adaptations of NADP-malic enzyme for its function in C4 photosynthesis in grasses (2019), Nat. Plants, 5, 755-765 .
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.2 M potassium fluoride and 30% (w/v) PEG 3350 Sorghum bicolor
sitting drop vapor diffusion method, using 0.1 M potassium chloride, 0.1 M sodium citrate (pH 5.5) and 34% (w/v) pentaerythritol propoxylate Zea mays

Protein Variants

Protein Variants Comment Organism
A339E the mutant of isoform nonC4-NADP-ME shows increased catalytic efficiency compared to the wild type enzyme Zea mays
DelN the mutant of isoform nonC4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme and is not inhibited by (S)-malate Zea mays
DelN/A339E the mutant of isoform nonC4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme Zea mays
DelN/I140F/A339E the mutant of isoform nonC4-NADP-ME shows increased catalytic efficiency compared to the wild type enzyme and is not inhibited by (S)-malate Zea mays
E339A the mutant of isoform C4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme and is not inhibited by (S)-malate Zea mays
F140I the mutant of isoform C4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme Zea mays
I140F the mutant of isoform nonC4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme and is not inhibited by (S)-malate Zea mays
I140F/A339E the mutant of isoform nonC4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme and is not inhibited by (S)-malate Zea mays
L544F the mutant of isoform C4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme Zea mays
Q503E the mutant of isoform C4-NADP-ME shows reduced catalytic efficiency compared to the wild type enzyme Zea mays

Inhibitors

Inhibitors Comment Organism Structure
(S)-malate
-
Sorghum bicolor
(S)-malate
-
Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0082
-
NADP+ mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.0128
-
NADP+ mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.0131
-
NADP+ mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.0149
-
NADP+ wild type isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.015
-
NADP+ mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.0159
-
NADP+ mutant E339A of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.0159
-
NADP+ mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.023
-
NADP+ mutant DelN of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.023
-
NADP+ wild type isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.038
-
NADP+ mutant I140F of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.0412
-
NADP+ mutant I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.0503
-
NADP+ mutant DelN/I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.23
-
(S)-malate wild type isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.27
-
(S)-malate mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.4
-
(S)-malate mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.43
-
(S)-malate wild type isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.46
-
(S)-malate mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.47
-
(S)-malate mutant E339A of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.47
-
(S)-malate mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.58
-
(S)-malate mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.85
-
(S)-malate mutant I140F of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
0.95
-
(S)-malate mutant DelN of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.05
-
(S)-malate mutant DelN/I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
1.12
-
(S)-malate mutant I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Zea mays 9507
-
chloroplast
-
Sorghum bicolor 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Zea mays
-
pyruvate + CO2 + NADPH + H+
-
r
(S)-malate + NADP+ Sorghum bicolor
-
pyruvate + CO2 + NADPH + H+
-
r
pyruvate + CO2 + NADPH + H+ Zea mays
-
(S)-malate + NADP+
-
r
pyruvate + CO2 + NADPH + H+ Sorghum bicolor
-
(S)-malate + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Sorghum bicolor
-
cultivar v.3.1.1
-
Zea mays
-
cultivar Ensembl-18
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Zea mays
Ni-NTA agarose column chromatography Sorghum bicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Zea mays pyruvate + CO2 + NADPH + H+
-
r
(S)-malate + NADP+
-
Sorghum bicolor pyruvate + CO2 + NADPH + H+
-
r
pyruvate + CO2 + NADPH + H+
-
Zea mays (S)-malate + NADP+
-
r
pyruvate + CO2 + NADPH + H+
-
Sorghum bicolor (S)-malate + NADP+
-
r

Subunits

Subunits Comment Organism
homotetramer 4 * 66000, SDS-PAGE Zea mays
homotetramer 4 * 66000, SDS-PAGE Sorghum bicolor

Synonyms

Synonyms Comment Organism
C4-NADP-ME isoform Zea mays
C4-NADP-ME isoform Sorghum bicolor
NADP-malic enzyme
-
Zea mays
NADP-malic enzyme
-
Sorghum bicolor
nicotinamide adenine dinucleotide phosphate-dependent malic enzyme
-
Zea mays
nicotinamide adenine dinucleotide phosphate-dependent malic enzyme
-
Sorghum bicolor
nonC4-NADP-ME isoform Zea mays
nonC4-NADP-ME isoform Sorghum bicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6
-
(S)-malate mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
4
-
(S)-malate mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
22
-
(S)-malate mutant DelN of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
26.3
-
(S)-malate wild type isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
27.4
-
(S)-malate mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
28.1
-
(S)-malate wild type isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
28.7
-
(S)-malate mutant E339A of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
29.8
-
(S)-malate mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
32.1
-
(S)-malate mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
36.7
-
(S)-malate mutant I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
39.6
-
(S)-malate mutant I140F of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
85.8
-
(S)-malate mutant DelN/I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Zea mays
7.5 8
-
Sorghum bicolor

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
9.1
-
(S)-malate wild type isoform C4-NADP-ME, at pH 8.0 and 25°C Zea mays
13.3
-
(S)-malate mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
13.4
-
(S)-malate mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
15.8
-
(S)-malate mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
16.7
-
(S)-malate mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
19.4
-
(S)-malate mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.7
-
(S)-malate mutant F140I of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
10
-
(S)-malate mutant DelN/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
23.2
-
(S)-malate mutant DelN of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
32.8
-
(S)-malate mutant I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
46.6
-
(S)-malate mutant I140F of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
47.2
-
(S)-malate mutant L544F of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
61.1
-
(S)-malate mutant E339A of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
62.6
-
(S)-malate wild type isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
64.8
-
(S)-malate mutant Q503E of isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays
81.7
-
(S)-malate mutant DelN/I140F/A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
118.9
-
(S)-malate mutant A339E of isoform nonC4-NADP-ME, at pH 7.0 and 25°C Zea mays
122.2
-
(S)-malate wild type isoform C4-NADP-ME, at pH 7.0 and 25°C Zea mays