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Literature summary for 1.1.1.40 extracted from

  • Mondal, D.K.; Pal, D.S.; Abbasi, M.; Datta, R.
    Functional partnership between carbonic anhydrase and malic enzyme in promoting gluconeogenesis in Leishmania major (2021), FEBS J., 288, 4129-4152 .
    View publication on PubMed
Search for more literature for 1.1.1.40

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Leishmania major

Inhibitors

Inhibitors Comment Organism Structure
3-(pyridin-2-yl)-6-[(5,6,7,8-tetrahydronaphthalen-2-yl)methyl][1,2,4]triazolo[3,4-b][1,3,4]thiadiazole ATR7-010 Leishmania major
additional information ATR4-003 (3-(4-methoxyphenyl)-2-methyl-5-([(4-methylpyrimidin-2-yl)sulfanyl]methyl)pyrazolo[1,5-a]pyrimidin-7(4H)-one) and ATR6-001 ([1-amino-5-(morpholin-4-yl)-6,7,8,9-tetrahydrothieno[2,3-c]isoquinolin-2-yl](piperidin-1-yl)methanone) do not inhibitenzyme activity up to a concentration of 0.02 mM Leishmania major

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Leishmania major 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 1 mM used in assay conditions Leishmania major

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Leishmania major
-
 pyruvate + CO2 + NADPH + H+
-
 r
pyruvate + CO2 + NADPH + H+ Leishmania major
-
 (S)-malate + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Leishmania major
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Leishmania major

Source Tissue

Source Tissue Comment Organism Textmining
amastigote
-
 Leishmania major
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
 Leishmania major pyruvate + CO2 + NADPH + H+
-
 r
pyruvate + CO2 + NADPH + H+
-
 Leishmania major (S)-malate + NADP+
-
 r

Subunits

Subunits Comment Organism
? x * 63400, SDS-PAGE Leishmania major

Synonyms

Synonyms Comment Organism
mitochondrial malic enzyme
-
 Leishmania major

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Leishmania major
NADPH
-
 Leishmania major

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00156
-
 pyruvate carboxylation, pH and temperature not specified in the publication Leishmania major 3-(pyridin-2-yl)-6-[(5,6,7,8-tetrahydronaphthalen-2-yl)methyl][1,2,4]triazolo[3,4-b][1,3,4]thiadiazole
0.00159
-
 malate decarboxylation, pH and temperature not specified in the publication Leishmania major 3-(pyridin-2-yl)-6-[(5,6,7,8-tetrahydronaphthalen-2-yl)methyl][1,2,4]triazolo[3,4-b][1,3,4]thiadiazole

Expression

Organism Comment Expression
Leishmania major the enzyme's protein level is strongly upregulated (more than 2fold) under low glucose conditions up

General Information

General Information Comment Organism
physiological function the enzyme is important for survival of Leishmania amastigotes within host macrophages Leishmania major