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Literature summary for 1.1.1.40 extracted from

  • Hasan, N.M.; Longacre, M.J.; Stoker, S.W.; Kendrick, M.A.; MacDonald, M.J.
    Mitochondrial malic enzyme 3 is important for insulin secretion in pancreatic beta-cells (2015), Mol. Endocrinol., 29, 396-410.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.40

Protein Variants

Protein Variants Comment Organism
additional information generation of several ME3 knockdown cell lines, knockdown of inhibits insulin release. In the Me3 double-knockdown cells, the level of Me1 mRNA is not significantly decreased in the Me3-628(P)/Me2-725(H) and Me3-1672(P)/Me2-725(H) cell lines. However, a 32%, 49%, and 47% decrease in ME1 activity is observed in the cell lines Me3-628(P), Me3-628(P)/Me2-725(H), and Me3-628(P)/Me2-2124(H), respectively. Me3 mRNA is decreased in the Me3 single-knockdown cell line Me3-628(P), and it is lowered further in the Me3 and Me1 double-knockdown Me3–628(P)/Me1-753(H) but not Me3-1672(P)/Me1-753(H). The level of Me3 mRNA in Me3-628(P)/Me1-753(H) and Me3-1672(P)/Me1-753(H) is 10% and 59% of the CHS control, respectively Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Rattus norvegicus
-
 pyruvate + CO2 + NADPH
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus F1M5N4
-
-

Source Tissue

Source Tissue Comment Organism Textmining
INS-1 832/13 cell
-
 Rattus norvegicus
-
insulinoma cell
-
 Rattus norvegicus
-
pancreas
-
 Rattus norvegicus
-
pancreatic beta cell ME3 enzyme activity is low in beta-cells Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
 Rattus norvegicus pyruvate + CO2 + NADPH
-
 ?

Synonyms

Synonyms Comment Organism
ME3
-
 Rattus norvegicus
mitochondrial NADP malic enzyme
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Rattus norvegicus
NADPH
-
 Rattus norvegicus

General Information

General Information Comment Organism
malfunction knockdown of ME3, but not ME1 or ME2 (both EC 1.1.1.39) alone or together, inhibits insulin release stimulated by glucose, pyruvate or 2-aminobicyclo [2,2,1]heptane-2-carboxylic acid-plus-glutamine Rattus norvegicus
physiological function mitochondrial NADP malic enzyme is necessary for insulin release Rattus norvegicus