Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mortierella alpina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | complete inhibition at 5 mM | Mortierella alpina | |
Co2+ | about 90% activity at 0.5 mM | Mortierella alpina | |
Cu2+ | complete inhibition at 5 mM | Mortierella alpina | |
glyoxylate | competitive inhibition, about 60% activity at 2 mM | Mortierella alpina | |
Mg2+ | about 80% activity at 0.5 mM | Mortierella alpina | |
additional information | not inhibited by ATP, ADP, AMP, propionyl-COA, acetyl-CoA, CoA, succinate, L-glutamate, L-aspartate, isocitrate, citrate, pyruvate, D-fructose 6-phosphate, D-glucose 6-phosphate, and 2-oxoglutarate | Mortierella alpina | |
Ni2+ | about 50% activity at 0.5 mM | Mortierella alpina | |
oxaloacetate | competitive inhibition, about 25% activity at 2 mM | Mortierella alpina | |
Zn2+ | about 10% activity at 5 mM | Mortierella alpina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
NADP+ | at pH 7.5 and 33°C | Mortierella alpina | |
2.19 | - |
(S)-malate | at pH 7.5 and 33°C | Mortierella alpina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | about 115% activity at 5 mM | Mortierella alpina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
x * 64000, SDS-PAGE | Mortierella alpina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Mortierella alpina | - |
pyruvate + CO2 + NADPH | - |
? | |
(S)-malate + NADP+ | Mortierella alpina ATCC 32222 | - |
pyruvate + CO2 + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mortierella alpina | - |
- |
- |
Mortierella alpina ATCC 32222 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Mortierella alpina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Mortierella alpina | pyruvate + CO2 + NADPH | - |
? | |
(S)-malate + NADP+ | - |
Mortierella alpina ATCC 32222 | pyruvate + CO2 + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, SDS-PAGE | Mortierella alpina |
Synonyms | Comment | Organism |
---|---|---|
malE1 | - |
Mortierella alpina |
NADP+-dependent malic enzyme | - |
Mortierella alpina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
33 | - |
the enzyme is completely inactivated above 45°C | Mortierella alpina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Mortierella alpina |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mortierella alpina |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
oxaloacetate | at pH 7.5 and 33°C | Mortierella alpina | |
0.6 | - |
glyoxylate | at pH 7.5 and 33°C | Mortierella alpina |