Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | PtNADP-ME1, PtNADP-ME3, and PtNADP-ME4 are activated | Populus trichocarpa | |
D-glucose 6-phosphate | activates PtNADP-ME3 and PtNADP-ME4 | Populus trichocarpa |
Cloned (Comment) | Organism |
---|---|
five NADP-ME genes PtNADP-ME1, PtNADP-ME2, PtNADP-ME3, PtNADP-ME4, and PtNADPME5, DNA and amino acid sequence determination and analysis, phylogenetic analysis, individual expression of GST-tagged isozymes in Escherichia coli | Populus trichocarpa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Citric acid | inhibits all PtNADP-ME activities significantly | Populus trichocarpa | |
CoA | activities of PtNADP-ME1, PtNADP-ME2 and PtNADPME4 proteins are inhibited | Populus trichocarpa | |
D-fructose | PtNADP-ME1 | Populus trichocarpa | |
oxaloacetic acid | inhibits all PtNADP-ME activities significantly | Populus trichocarpa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | among the five isoforms, PtNADP-ME5 has the highest affinities for NADP while PtNADP-ME2 exhibits the lowest affinities toward both malate and NADP+ | Populus trichocarpa | |
0.03 | - |
NADP+ | recombinant isozyme PtNADP-ME5, pH 7.5, 30°C | Populus trichocarpa | |
0.036 | - |
NADP+ | recombinant isozyme PtNADP-ME1, pH 7.5, 30°C | Populus trichocarpa | |
0.075 | - |
NADP+ | recombinant isozyme PtNADP-ME4, pH 7.5, 30°C | Populus trichocarpa | |
0.1 | - |
NADP+ | recombinant isozyme PtNADP-ME3, pH 7.5, 30°C | Populus trichocarpa | |
0.12 | - |
NADP+ | recombinant isozyme PtNADP-ME2, pH 7.5, 30°C | Populus trichocarpa | |
1 | - |
(S)-malate | recombinant isozyme PtNADP-ME1, pH 7.5, 30°C | Populus trichocarpa | |
1.1 | - |
(S)-malate | recombinant isozyme PtNADP-ME5, pH 7.5, 30°C | Populus trichocarpa | |
1.4 | - |
(S)-malate | recombinant isozyme PtNADP-ME4, pH 7.5, 30°C | Populus trichocarpa | |
1.5 | - |
(S)-malate | recombinant isozyme PtNADP-ME3, pH 7.5, 30°C | Populus trichocarpa | |
2.9 | - |
(S)-malate | recombinant isozyme PtNADP-ME2, pH 7.5, 30°C | Populus trichocarpa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | putative plastidic isozymes PtNADP-ME4 and PtNADP-ME5 | Populus trichocarpa | 9507 | - |
cytosol | isozymes PtNADP-ME2 and PtNADPME3 | Populus trichocarpa | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Populus trichocarpa | - |
pyruvate + CO2 + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus trichocarpa | - |
five NADP-ME genes, PtNADP-ME1, PtNADP-ME2, PtNADP-ME3, PtNADP-ME4, and PtNADPME5 | - |
Purification (Comment) | Organism |
---|---|
recombinant isozymes PtNADP-ME1, PtNADP-ME2, PtNADP-ME3, PtNADP-ME4, and PtNADPME5 from Escherichia coli by glutathione affinity chromatography | Populus trichocarpa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | transcription levels of PtNADP-ME1 in lignified stems and roots are clearly higher than in other tissues, and PtNADP-ME2, PtNADP-ME3, PtNADP-ME4 and PtNADPME5 are broadly expressed in various tissues | Populus trichocarpa | - |
root | - |
Populus trichocarpa | - |
stem | lighified | Populus trichocarpa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Populus trichocarpa | pyruvate + CO2 + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | the cytosolic PtNADP-ME2 aggregates as octamers and hexadecamers while the plastidic PtNADP-ME4 resembles hexamers and octamers. Different PtNADP-ME family members present different oligomeric states in vitro | Populus trichocarpa |
Synonyms | Comment | Organism |
---|---|---|
NADP-malic enzyme | - |
Populus trichocarpa |
NADP-ME | - |
Populus trichocarpa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Populus trichocarpa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
NADP+ | recombinant isozyme PtNADP-ME1, pH 7.5, 30°C | Populus trichocarpa | |
0.9 | - |
(S)-malate | recombinant isozyme PtNADP-ME1, pH 7.5, 30°C | Populus trichocarpa | |
12.6 | - |
(S)-malate | recombinant isozyme PtNADP-ME2, pH 7.5, 30°C | Populus trichocarpa | |
13.2 | - |
NADP+ | recombinant isozyme PtNADP-ME2, pH 7.5, 30°C | Populus trichocarpa | |
13.6 | - |
(S)-malate | recombinant isozyme PtNADP-ME3, pH 7.5, 30°C | Populus trichocarpa | |
14.2 | - |
NADP+ | recombinant isozyme PtNADP-ME3, pH 7.5, 30°C | Populus trichocarpa | |
15.7 | - |
(S)-malate | recombinant isozyme PtNADP-ME4, pH 7.5, 30°C | Populus trichocarpa | |
15.9 | - |
NADP+ | recombinant isozyme PtNADP-ME4, pH 7.5, 30°C | Populus trichocarpa | |
19.1 | - |
(S)-malate | recombinant isozyme PtNADP-ME5, pH 7.5, 30°C | Populus trichocarpa | |
19.5 | - |
NADP+ | recombinant isozyme PtNADP-ME5, pH 7.5, 30°C | Populus trichocarpa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Populus trichocarpa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Populus trichocarpa |
Organism | Comment | Expression |
---|---|---|
Populus trichocarpa | significant decrease of PtNADP-ME2 and PtNADP-ME3 in mRNA levels, but only a slight change in other PtNADP-ME genes, during the dark | down |
Populus trichocarpa | NaCl salt stress upregulates the transcripts of putative plastidic isozymes PtNADP-ME4 and PtNADP-ME5 significantly. Expression of PtNADP-ME2 and PtNADP-ME3 increases during the course of leaf wounding, PtNADP-ME3 transcript levels peak at 6 h under NaCl and mannitol stresses. Expression of PtNADP-ME1 gene is induced slightly by mannitol and PEG osmotic stresses, but not by salt stress | up |
General Information | Comment | Organism |
---|---|---|
evolution | the five PtNADP-ME isoforms cluster in a phylogenetic tree constructed with the whole set of plant NADP-ME sequences, classification into four groups. PtNADP-ME2 and PtNADPME3 cluster with the cytosolic dicot NADP-ME group (group II), while PtNADP-ME4 and PtNADP-ME5 are included in the plastidic dicot NADP-ME group (group III). The group IV comprises both monocot and dicot enzymes, including PtNADP-ME1. Neither of the PtNADP-ME isoforms is included in the monocot NADPMEs (group I) | Populus trichocarpa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
(S)-malate | recombinant isozyme PtNADP-ME1, pH 7.5, 30°C | Populus trichocarpa | |
4.3 | - |
(S)-malate | recombinant isozyme PtNADP-ME2, pH 7.5, 30°C | Populus trichocarpa | |
9.1 | - |
(S)-malate | recombinant isozyme PtNADP-ME3, pH 7.5, 30°C | Populus trichocarpa | |
11 | - |
(S)-malate | recombinant isozyme PtNADP-ME4, pH 7.5, 30°C | Populus trichocarpa | |
22 | - |
NADP+ | recombinant isozyme PtNADP-ME1, pH 7.5, 30°C | Populus trichocarpa | |
110 | - |
NADP+ | recombinant isozyme PtNADP-ME2, pH 7.5, 30°C | Populus trichocarpa | |
140 | - |
NADP+ | recombinant isozyme PtNADP-ME3, pH 7.5, 30°C | Populus trichocarpa | |
170 | - |
(S)-malate | recombinant isozyme PtNADP-ME5, pH 7.5, 30°C | Populus trichocarpa | |
210 | - |
NADP+ | recombinant isozyme PtNADP-ME4, pH 7.5, 30°C | Populus trichocarpa | |
650 | - |
NADP+ | recombinant isozyme PtNADP-ME5, pH 7.5, 30°C | Populus trichocarpa |