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Literature summary for 1.1.1.40 extracted from

  • Hsieh, J.Y.; Hung, H.C.
    Engineering of the cofactor specificities and isoform-specific inhibition of malic enzyme (2009), J. Biol. Chem., 284, 4536-4544.
    View publication on PubMed
Search for more literature for 1.1.1.40

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ATP weak inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
 NADP+ wild type enzyme, in 50 mMTris-HCl (pH 7.4), at 30°C Homo sapiens
11.5
-
 NAD+ wild type enzyme, in 50 mMTris-HCl (pH 7.4), at 30°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity gel column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+ low activity with NAD+ as cofactor Homo sapiens pyruvate + CO2 + NADH
-
 ?
(S)-malate + NADP+
-
 Homo sapiens pyruvate + CO2 + NADPH
-
 ?

Synonyms

Synonyms Comment Organism
c-NADP-ME
-
 Homo sapiens
cytosolic NADP+-dependent malic enzyme
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
28.9
-
 NAD+ wild type enzyme, in 50 mMTris-HCl (pH 7.4), at 30°C Homo sapiens
135.8
-
 NADP+ wild type enzyme, in 50 mMTris-HCl (pH 7.4), at 30°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
17.27
-
 ATP wild type enzyme, in 50 mMTris-HCl (pH 7.4), at 30°C Homo sapiens