Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fumarate | about 4fold increase of activity in the presence of 7.5 mM fumarate | Arabidopsis thaliana | |
succinate | about 3fold increase of activity in the presence of 7.5 mM fumarate | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Arabidopsis thaliana | |
aspartate | - |
Arabidopsis thaliana | |
ATP | - |
Arabidopsis thaliana | |
D-Glucose-6-phosphate | - |
Arabidopsis thaliana | |
fumarate | - |
Arabidopsis thaliana | |
oxaloacetate | - |
Arabidopsis thaliana | |
succinate | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
NADP+ | pH 7.5 | Arabidopsis thaliana | |
0.072 | - |
NADP+ | pH 7.5 | Arabidopsis thaliana | |
0.5 | - |
pyruvate | pH 7.0 | Arabidopsis thaliana | |
0.8 | - |
(S)-malate | pH 7.5 | Arabidopsis thaliana | |
3.3 | - |
(S)-malate | pH 7.5 | Arabidopsis thaliana | |
5 | - |
pyruvate | pH 7.0 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
SDS-PAGE | Arabidopsis thaliana |
243000 | - |
gel filtration | Arabidopsis thaliana |
249000 | - |
gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LYG3 | - |
- |
Arabidopsis thaliana | Q9XGZ0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Arabidopsis thaliana | pyruvate + CO2 + NADPH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NADP-malic enzyme | - |
Arabidopsis thaliana |
NADP-ME2 | isoform | Arabidopsis thaliana |
NADP-ME3 | isoform | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
75 | - |
pyruvate | pH 7.0 | Arabidopsis thaliana | |
237 | - |
pyruvate | pH 7.0 | Arabidopsis thaliana | |
268 | - |
(S)-malate | pH 7.5 | Arabidopsis thaliana | |
324 | - |
(S)-malate | pH 7.5 | Arabidopsis thaliana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
at pH 7.5 isoenzyme NADP-ME2 retains 95% of the maximal activity | Arabidopsis thaliana |
7.5 | - |
at pH 7.5 isoenzyme NADP-ME3 retains 95% of the maximal activity | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Arabidopsis thaliana |