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Literature summary for 1.1.1.40 extracted from

  • Bartolucci, S.; Rella, R.; Guagliardi, A.; Raia, C.A.; Gambacorta, A.; De Rosa, M.; Rossi, M.
    Malic enzyme from archaebacterium Sulfolobus solfataricus. Purification, structure, and kinetic properties. (1987), J. Biol. Chem., 262, 7725-7731.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
NADP+ 60°C, pH 8.0 Saccharolobus solfataricus
0.018
-
(S)-malate 60°C, pH 8.0, presence of Mn2+ Saccharolobus solfataricus
0.065
-
(S)-malate 60°C, pH 8.0, presence of Mg2+ Saccharolobus solfataricus
0.25
-
NAD+ 60°C, pH 8.0 Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ may partly replace for Mn2+ Saccharolobus solfataricus
Mn2+ most effective divalent cation Saccharolobus solfataricus
additional information absolute requirement for a divalent cation. Ca2+ is barely effective Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
2 * 49000, SDS-PAGE Saccharolobus solfataricus
105000
-
gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
enzyme additionally catalyzes decarboxylation of oxalacetate
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36.6
-
60°C, pH 8.0 Saccharolobus solfataricus

Storage Stability

Storage Stability Organism
25°C, pH 8.0, stable for at least one month Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+ NADP+ is preferred over NAD+ Saccharolobus solfataricus pyruvate + CO2 + NADH
-
?
(S)-malate + NADP+ NADP+ is preferred over NAD+ Saccharolobus solfataricus pyruvate + CO2 + NADPH
-
?
additional information enzyme additionally catalyzes decarboxylation of oxalacetate Saccharolobus solfataricus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 49000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
stable for at least 24 h Saccharolobus solfataricus
75
-
stable for at least 12 h Saccharolobus solfataricus
80
-
stable for at least 7 h Saccharolobus solfataricus
85
-
stable for at least 5 h Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
376
-
(S)-malate pH 8.0, 60°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Saccharolobus solfataricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
stable for 24 h Saccharolobus solfataricus
8
-
stable for at least one month Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Saccharolobus solfataricus
NADP+
-
Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus isoelectric focusing
-
5.1