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Literature summary for 1.1.1.40 extracted from

  • Yang, Z.; Zhang, H.; Hung, H.C.; Kuo, C.C.; Tsai, L.C.; Yuan, H.S.; Chou, W.Y.; Chang, G.G.; Tong, L.
    Structural studies of the pigeon cytosolic NADP(+)-dependent malic enzyme (2002), Protein Sci., 11, 332-341.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Columba sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
10 mg/ml purified enzyme in quarternary complex with NADP+, Mn2+, and oxalate, closed enzyme form, hanging drop vapour diffusion method, 4°C, from 20 mM Tris, pH 7.4, 10 mM oxalate, 5 mM MnCl2, 0.23 mM NADP+, and 2 mM DTT, with a reservoir solution containing 100 mM sodium citrate, pH 5.5, 8% PEG 6000, and 1 M LiCl, 3-4 days, X-ray diffraction structure determination and analysis at 2.5 A resolution, atomic model Columba sp.

Protein Variants

Protein Variants Comment Organism
K362A site-directed mutagenesis, 70fold increased Km for NADP+ compared to the wild-type enzyme Columba sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Columba sp. 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Columba sp.

Organism

Organism UniProt Comment Textmining
Columba sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by pecipitation with streptomycin sulfate, DEAE ion exchange, affinity and gel filtration Columba sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Columba sp. pyruvate + CO2 + NADPH
-
r

Subunits

Subunits Comment Organism
tetramer subunit structure and organisation, crystal structure Columba sp.

Synonyms

Synonyms Comment Organism
malic enzyme
-
Columba sp.
ME
-
Columba sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Columba sp.

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on, 1 cofactor molecule per subunit, binding mode, structure around the binding site at the 2'-phosphate group helps to define the cofactor specificity of the enzyme, Lys362 and Ser346 are involved, molecular mechanism Columba sp.