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Literature summary for 1.1.1.40 extracted from

  • Eyzaguirre, J.; Cornwell, E.; Borie, G.; Ramirez, B.
    Two malic enzymes in Pseudomonas aeruginosa (1973), J. Bacteriol., 116, 215-221.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.40

Activating Compound

Activating Compound Comment Organism Structure
NH4+ activates Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 NADP+
-
 Pseudomonas aeruginosa
2.7
-
 (S)-malate
-
 Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates Pseudomonas aeruginosa
K+ Km: 0.7 mM Pseudomonas aeruginosa
Mg2+ divalent metal ion required, Mn2+ is more effective than Mg2+, Km: 0.34 mM Pseudomonas aeruginosa
Mn2+ Km: 0.004 mM Pseudomonas aeruginosa
Mn2+ divalent metal ion required, Mn2+ is more effective than Mg2+ Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 gel filtration Pseudomonas aeruginosa
90000
-
 sucrose density gradient centrifugation Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
 Pseudomonas aeruginosa pyruvate + CO2 + NADPH
-
 r
pyruvate + CO2 + NADPH
-
 Pseudomonas aeruginosa L-malate + NADP+
-
 r

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Pseudomonas aeruginosa