Cloned (Comment) | Organism |
---|---|
gene bdhA, recombinant eenzyme expression in non-natural AC and 2,3-BD producing Corynebacterium crenatum derived from wild-type strain SYPA5-5, coexpression of 2,3-BD biosynthesis pathway genes alsS, alsD, and bdhA | Bacillus subtilis subsp. subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | coexpression of 2,3-BD biosynthesis pathway genes alsS, alsD, and bdhA in synthetically engineered strains Corynebacterium crenatumDELTAbutADELTAldh SD and Corynebacterium crenatumDELTAldh SDA for selective acetoin (AC) and 2,3-butanediol (2,3-BD) production. Production of AC and 2,3-BD using resting cell bioconversion with glucose as substrate, method evaluation, overview | Bacillus subtilis subsp. subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,R)-butane-2,3-diol + NAD+ | Bacillus subtilis subsp. subtilis | - |
(R)-acetoin + NADH + H+ | - |
r | |
(R,R)-butane-2,3-diol + NAD+ | Bacillus subtilis subsp. subtilis 168 | - |
(R)-acetoin + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis subsp. subtilis | O34788 | - |
- |
Bacillus subtilis subsp. subtilis 168 | O34788 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,R)-butane-2,3-diol + NAD+ | - |
Bacillus subtilis subsp. subtilis | (R)-acetoin + NADH + H+ | - |
r | |
(R,R)-butane-2,3-diol + NAD+ | - |
Bacillus subtilis subsp. subtilis 168 | (R)-acetoin + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
2,3-butanediol dehydrogenase | - |
Bacillus subtilis subsp. subtilis |
BdhA | - |
Bacillus subtilis subsp. subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
in vivo assay at | Bacillus subtilis subsp. subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bacillus subtilis subsp. subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis subsp. subtilis | |
NADH | - |
Bacillus subtilis subsp. subtilis |