KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
NAD+ | at pH 10.0 and 30°C | Rhodococcus sp. AD45 | |
1.4 | - |
1-hydroxy-2-glutathionyl-2-methyl-3-butene | at pH 10.0 and 30°C | Rhodococcus sp. AD45 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
2 * 25000, SDS-PAGE | Rhodococcus sp. AD45 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. AD45 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Blue Sepharose column chromatography, DE52 column chromatography, and Sephacryl S-300 gel filtration | Rhodococcus sp. AD45 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.9 | - |
crude extract, at pH 10.0 and 30°C | Rhodococcus sp. AD45 |
18 | - |
after 6.2fold purification, at pH 10.0 and 30°C | Rhodococcus sp. AD45 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-hydroxy-2-glutathionyl-2-methyl-3-butene + NAD+ | - |
Rhodococcus sp. AD45 | N-(2-methyl-1-oxobut-3-en-2-yl)-L-gamma-glutamyl-L-cysteinylglycine + NADH + H+ | - |
? | |
2-glutathionyl-2-methyl-3-butenal + NAD+ + H2O | - |
Rhodococcus sp. AD45 | 2-glutathionyl-2-methyl-3-butenoic acid + NADH + H+ | - |
? | |
additional information | no NADH formation is observed when GSH, methanol, ethanol, glycerol, 2-methylbutanol, 3-methyl-butanol, 1-hydroxy-3-methyl-3-butene, or 1,2-dihydroxy-2-methyl-3-butene are used as a substrate. The conjugates of GSH with 1,2-epoxybutane and 1,2-epoxyhexane are also not substrates | Rhodococcus sp. AD45 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 25000, SDS-PAGE | Rhodococcus sp. AD45 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | - |
Rhodococcus sp. AD45 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rhodococcus sp. AD45 |