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Literature summary for 1.1.1.398 extracted from

  • Van Hylckama Vlieg, J.; Kingma, J.; Kruizinga, W.; Janssen, D.
    Purification of a glutathione S-transferase and a glutathione conjugate-specific dehydrogenase involved in isoprene metabolism in Rhodococcus sp. strain AD45 (1999), J. Bacteriol., 181, 2094-2101.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
NAD+ at pH 10.0 and 30°C Rhodococcus sp. AD45
1.4
-
1-hydroxy-2-glutathionyl-2-methyl-3-butene at pH 10.0 and 30°C Rhodococcus sp. AD45

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
2 * 25000, SDS-PAGE Rhodococcus sp. AD45

Organism

Organism UniProt Comment Textmining
Rhodococcus sp. AD45
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Blue Sepharose column chromatography, DE52 column chromatography, and Sephacryl S-300 gel filtration Rhodococcus sp. AD45

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.9
-
crude extract, at pH 10.0 and 30°C Rhodococcus sp. AD45
18
-
after 6.2fold purification, at pH 10.0 and 30°C Rhodococcus sp. AD45

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hydroxy-2-glutathionyl-2-methyl-3-butene + NAD+
-
Rhodococcus sp. AD45 N-(2-methyl-1-oxobut-3-en-2-yl)-L-gamma-glutamyl-L-cysteinylglycine + NADH + H+
-
?
2-glutathionyl-2-methyl-3-butenal + NAD+ + H2O
-
Rhodococcus sp. AD45 2-glutathionyl-2-methyl-3-butenoic acid + NADH + H+
-
?
additional information no NADH formation is observed when GSH, methanol, ethanol, glycerol, 2-methylbutanol, 3-methyl-butanol, 1-hydroxy-3-methyl-3-butene, or 1,2-dihydroxy-2-methyl-3-butene are used as a substrate. The conjugates of GSH with 1,2-epoxybutane and 1,2-epoxyhexane are also not substrates Rhodococcus sp. AD45 ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 25000, SDS-PAGE Rhodococcus sp. AD45

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10
-
Rhodococcus sp. AD45

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rhodococcus sp. AD45