Literature summary for 1.1.1.390 extracted from

  • Felux A.-K, F.A.; Spiteller D, S.D.; Klebensberger J, K.J.; Schleheck D, S.D.
    Entner-Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1 (2015), Proc. Natl. Acad. Sci. USA, 112, E4298-E4305.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
sulfoquinovose with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida
2.4
-
sulfoquinovose with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas putida the enzyme shows no activity with D-glucose-6-phosphate ?
-
?
sulfoquinovose + NAD+ Pseudomonas putida sulfoquinovose is 6-deoxy-6-sulfoglucose 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
-
?
additional information Pseudomonas putida SQ1 the enzyme shows no activity with D-glucose-6-phosphate ?
-
?
sulfoquinovose + NAD+ Pseudomonas putida SQ1 sulfoquinovose is 6-deoxy-6-sulfoglucose 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida SQ1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows no activity with D-glucose-6-phosphate Pseudomonas putida ?
-
?
additional information the enzyme shows no activity with D-glucose-6-phosphate Pseudomonas putida SQ1 ?
-
?
sulfoquinovose + NAD+ sulfoquinovose is 6-deoxy-6-sulfoglucose Pseudomonas putida 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
-
?
sulfoquinovose + NAD+ sulfoquinovose is 6-deoxy-6-sulfoglucose Pseudomonas putida SQ1 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
-
?
sulfoquinovose + NADP+ when NADP+ is used as an electron acceptor, the enzyme activity is 4% of the activity with NAD+ Pseudomonas putida 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
-
?
sulfoquinovose + NADP+ when NADP+ is used as an electron acceptor, the enzyme activity is 4% of the activity with NAD+ Pseudomonas putida SQ1 6-deoxy-6-sulfo-D-glucono-1,5-lactone + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
SQ dehydrogenase
-
Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
sulfoquinovose with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida
33.8
-
sulfoquinovose with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Pseudomonas putida
NADP+ when NADP+ is used as an electron acceptor, the enzyme activity is 4% of the activity with NAD+ Pseudomonas putida

KCat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.6
-
sulfoquinovose with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida
67.6
-
sulfoquinovose with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication Pseudomonas putida