KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
NAD+ | - |
Tritrichomonas suis | |
0.1 | - |
(S)-malate | with NAD+ as cofactor | Tritrichomonas suis | |
0.207 | - |
NADP+ | - |
Tritrichomonas suis | |
0.458 | - |
(S)-malate | with NADP+ as cofactor | Tritrichomonas suis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
hydrogenosome | - |
Tritrichomonas suis | 42566 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation by Mn2+, at 1 mM, is 10% and 20% higher than activation with 1 mM Mg2+ in the presence of NAD+ and NADP+ | Tritrichomonas suis | |
Mg2+ | completely dependent on the presence of Mg2+ or Mn2+ | Tritrichomonas suis | |
Mn2+ | completely dependent on the presence of Mg2+ or Mn2+ | Tritrichomonas suis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
308000 | - |
gel filtration | Tritrichomonas suis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tritrichomonas suis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Tritrichomonas suis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.5 | - |
- |
Tritrichomonas suis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Tritrichomonas suis | pyruvate + CO2 + NADPH | - |
? | |
malate + NAD+ | - |
Tritrichomonas suis | pyruvate + CO2 + NADH + H+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cofactor | Tritrichomonas suis | |
NADP+ | activity is maximal 65% of the activity with NAD+ | Tritrichomonas suis |