Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.386 extracted from

  • Figueroa-Teran, R.; Pak, H.; Blomquist, G.J.; Tittiger, C.
    High substrate specificity of ipsdienol dehydrogenase (IDOLDH), a short-chain dehydrogenase from Ips pini bark beetles (2016), J. Biochem., 160, 141-151 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.386

Cloned(Commentary)

Cloned (Comment) Organism
gene IDOLDH, recombinant expression in Spodoptera frugiperda Sf9 cells via baculovirus transfection method Ips pini

Inhibitors

Inhibitors Comment Organism Structure
(-)-menthone
-
 Ips pini
1,10-phenanthroline
-
 Ips pini
1-propanol
-
 Ips pini
2-propanol
-
 Ips pini
ipsdienone
-
 Ips pini
additional information 1,3-butanediol, nerol, geraniol, citral, (S)-ipsdienol do not act as inhibitors Ips pini

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, recombinant enzyme Ips pini
0.000008
-
 NADP+ pH 7.5, 25°C, recombinant enzyme, with (R)-ipsdienol Ips pini
0.002
-
 ipsdienone pH 7.5, 25°C, recombinant enzyme Ips pini
0.003
-
 ipsenone pH 7.5, 25°C, recombinant enzyme Ips pini
0.009
-
 racemic ipsenol pH 7.5, 25°C, recombinant enzyme Ips pini
0.527
-
 (R)-ipsdienol pH 7.5, 25°C, recombinant enzyme Ips pini

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
117000
-
 recombinant enzyme, gel filtration Ips pini

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-ipsdienol + NADP+ Ips pini stereospecific reaction ipsdienone + NADPH + H+
-
 r
racemic ipsenol + NADP+ Ips pini
-
 ipsenone + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Ips pini G5DGA8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme about 2.5fold from Sf9 cells by ammonium sulfate fractionation Ips pini

Source Tissue

Source Tissue Comment Organism Textmining
additional information IDOLDH expression pattern, overview Ips pini
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.05
-
 partially purified recombinant enzyme, pH 7.5, 25°C, substrate ipsdienone Ips pini
0.092
-
 partially purified recombinant enzyme, pH 7.5, 25°C, substrate ipsenone Ips pini
0.196
-
 partially purified recombinant enzyme, pH 7.5, 25°C, substrate racemic ipsenol Ips pini
0.52 0.525 partially purified recombinant enzyme, pH 7.5, 25°C, Tris-HCl or phosphate buffer, substrate (R)-ipsdienol Ips pini

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-ipsdienol + NADP+ stereospecific reaction Ips pini ipsdienone + NADPH + H+
-
 r
(R)-ipsdienol + NADP+ stereospecific reaction, no activity with (S)-ipsdienol. The oxidation of (R)-ipsdienol is 6.3times higher than that for the reduction of ipsdienone Ips pini ipsdienone + NADPH + H+
-
 r
ipsdienone + NADPH + H+ stereospecific reaction Ips pini (R)-ipsdienol + NADP+
-
 r
ipsenone + NADPH + H+
-
 Ips pini racemic ipsenol + NADP+
-
 r
additional information the enzyme stereospecifically reduces both ketones to their corresponding (R)-alcohols. Low activity with (R)-menthone, 3-penten-2-ol, 17beta-estradiol, poor or no activity with (S)-ipsdienol, nerol, geraniol, myrcene, citral, 4-methyl-3-penten-2-one, 4-methyl-2-pentanone, 4-methyl-2-pentanol, acetone, 1,3-butendiol, etanol, 2-propanol, 3-hydroxybutyrate, and 2-oxobutyrate, substrate specificity, overview. Product identification by GC-MS analysis Ips pini ?
-
 ?
racemic ipsenol + NADP+
-
 Ips pini ipsenone + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
homotetramer 4 * 27500, recombinant enzyme, SDS-PAGE, functional enzyme form Ips pini

Synonyms

Synonyms Comment Organism
IDOLDH
-
 Ips pini

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Ips pini

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Ips pini

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Ips pini
NADPH
-
 Ips pini

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.015
-
 ipsdienone pH 7.5, 25°C, recombinant enzyme Ips pini
0.03
-
 (-)-menthone pH 7.5, 25°C, recombinant enzyme Ips pini
9.9
-
 2-propanol pH 7.5, 25°C, recombinant enzyme Ips pini
33.6
-
 1-propanol pH 7.5, 25°C, recombinant enzyme Ips pini

General Information

General Information Comment Organism
evolution the enzyme belongs to the short-chain oxidoreductase ipsdienol dehydrogenases Ips pini
additional information the enzyme contains the typical Rossmann-fold motif for nucleotide binding and the catalytic tetrad that are highly conserved Ips pini
physiological function Ips spp. bark beetles use ipsdienol, ipsenol, ipsdienone and ipsenone as aggregation pheromone components and pheromone precursors. For Ips pini, the short-chain oxidoreductase ipsdienol dehydrogenase (IDOLDH) converts (R)-ipsdienol to ipsdienone, and thus likely plays a role in determining pheromone composition. Different IDOLDH orthologues and or activity levels contribute to differences in Ips spp. pheromone composition Ips pini