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Literature summary for 1.1.1.383 extracted from

  • Brinkmann-Chen, S.; Cahn, J.K.; Arnold, F.H.
    Uncovering rare NADH-preferring ketol-acid reductoisomerases (2014), Metab. Eng., 26C, 17-22.
    View publication on PubMed
Search for more literature for 1.1.1.383

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Metallosphaera sedula
expression in Escherichia coli Hydrogenobaculum sp. Y04AAS1
expression in Escherichia coli Ignisphaera aggregans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 NADH Km-value for NADH is below 0.001 mM, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Ignisphaera aggregans
additional information
-
 NADPH Km-value for NADPH is below 0.001 mM, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Ignisphaera aggregans
0.024
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Metallosphaera sedula
0.031
-
 NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Metallosphaera sedula
0.039
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Hydrogenobaculum sp. Y04AAS1
0.046
-
 NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Hydrogenobaculum sp. Y04AAS1

Organism

Organism UniProt Comment Textmining
Hydrogenobaculum sp. Y04AAS1
-
-
-
Ignisphaera aggregans
-
-
-
Metallosphaera sedula
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Metallosphaera sedula
-
 Hydrogenobaculum sp. Y04AAS1
-
 Ignisphaera aggregans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate Metallosphaera sedula (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
 ?
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate Hydrogenobaculum sp. Y04AAS1 (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
 ?
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate Ignisphaera aggregans (2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
 ?
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate Metallosphaera sedula (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
-
 ?
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate Hydrogenobaculum sp. Y04AAS1 (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
-
 ?
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ (2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate Ignisphaera aggregans (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Ignisphaera aggregans
0.03
-
 NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Ignisphaera aggregans
0.06
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Metallosphaera sedula
0.07
-
 NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Metallosphaera sedula
0.12
-
 NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Hydrogenobaculum sp. Y04AAS1
0.12
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Hydrogenobaculum sp. Y04AAS1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Metallosphaera sedula
7
-
 assay at Hydrogenobaculum sp. Y04AAS1
7
-
 assay at Ignisphaera aggregans

Cofactor

Cofactor Comment Organism Structure
NADH the enzyme can use both NADH and NADPH with similar efficiency Metallosphaera sedula
NADH the enzyme can use both NADH and NADPH with similar efficiency Hydrogenobaculum sp. Y04AAS1
NADH the enzyme can use both NADH and NADPH with similar efficiency Ignisphaera aggregans
NADPH the enzyme can use both NADH and NADPH with similar efficiency Metallosphaera sedula
NADPH the enzyme can use both NADH and NADPH with similar efficiency Hydrogenobaculum sp. Y04AAS1
NADPH the enzyme can use both NADH and NADPH with similar efficiency Ignisphaera aggregans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 NADH kcat/Km-value for NADH is above 20/mM*s, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Ignisphaera aggregans
additional information
-
 NADPH kcat/Km-value for NADPH is above 25/mM*s, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Ignisphaera aggregans
2.1
-
 NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Metallosphaera sedula
2.5
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Metallosphaera sedula
2.7
-
 NADPH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Hydrogenobaculum sp. Y04AAS1
3.2
-
 NADH pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate Hydrogenobaculum sp. Y04AAS1