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Literature summary for 1.1.1.38 extracted from

  • Hasan, N.M.; Longacre, M.J.; Stoker, S.W.; Kendrick, M.A.; MacDonald, M.J.
    Mitochondrial malic enzyme 3 is important for insulin secretion in pancreatic beta-cells (2015), Mol. Endocrinol., 29, 396-410.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.38

Cloned(Commentary)

Cloned (Comment) Organism
gene ME2, quantitative RT-PCR expression analysis of isozymes Me1, Me2, and Me3 in wild-type strain and mutant Me2 strains Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information generation of Me2 single and double knockdown cell lines, thereby generating cel lines Me2–2124(H)/Me2–2124(P) and Me2–2124(H)/Me2-654(P), overview. Me2 mRNA and ME2 enzyme activity are drastically reduced in the double-knockdown cell lines Me2-2124(H)/Me2-2124(P) and Me2-2124(H)/Me2-456(P). Me3 mRNA is slightly reduced in these two cell lines, whereas Me1 mRNA and ME1 enzyme activity are not affected. Resident siRNA might interfere with the expression of the siRNA expressed from the second vector. Knockdown of isozyme ME3, EC 1.1.1.40, but not ME1 or ME2 (both EC 1.1.1.39) alone or together, inhibits insulin release stimulated by glucose, pyruvate or 2-aminobicyclo [2,2,1]heptane-2-carboxylic acid-plus-glutamine Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Rattus norvegicus
-
 pyruvate + CO2 + NADH
-
 ?
(S)-malate + NADP+ Rattus norvegicus
-
 pyruvate + CO2 + NADPH
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus A0A0G2K502
-
-

Source Tissue

Source Tissue Comment Organism Textmining
INS-1 832/13 cell
-
 Rattus norvegicus
-
insulinoma cell
-
 Rattus norvegicus
-
pancreas
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Rattus norvegicus pyruvate + CO2 + NADH
-
 ?
(S)-malate + NADP+
-
 Rattus norvegicus pyruvate + CO2 + NADPH
-
 ?

Synonyms

Synonyms Comment Organism
ME2
-
 Rattus norvegicus
mitochondrial NAD(P) malic enzyme
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Rattus norvegicus
NADH
-
 Rattus norvegicus
NADP+
-
 Rattus norvegicus
NADPH
-
 Rattus norvegicus

General Information

General Information Comment Organism
malfunction knockdown of ME2 does not inhibit insulin release stimulated by glucose, pyruvate or 2-aminobicyclo [2,2,1]heptane-2-carboxylic acid-plus-glutamine Rattus norvegicus