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Literature summary for 1.1.1.379 extracted from
- Biosynthesis of phenylglyoxylic acid by LhDMDH, a novel D-mandelate dehydrogenase with high catalytic activity (2018), J. Agric. Food Chem., 66, 2805-2811 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | D-mandelate dehydrogenase (DMDH) has the potential to convert D-mandelic acid to phenylglyoxylic acid (PGA), which is a key building block in the field of chemical synthesis and is widely used to synthesize pharmaceutical intermediates or food additives. Development of an alternative strategy for the chiral resolution of racemic mandelic acid and the biosynthesis of PGA | Schleiferilactobacillus harbinensis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cloning, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21, coexpression with LDH from Lactobacillus casei increasing the phenylglyoxylate yield | Schleiferilactobacillus harbinensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | by coculturing two Escherichia coli strains expressing LhDMDH and lactate dehydrogenase (LDH) from Lactobacillus casei, LcLDH, a system for the efficient synthesis of phenylglyoxylic acid (PGA) is developed, achieving a 60% theoretical yield and 99% purity without adding coenzyme or cosubstrate. Implementation of a promising strategy for the chiral resolution of racemic mandelic acid and the biosynthesis of PGA. Whole cell catalysis is a preferred option to eliminate the influence of low thermostability of enzyme LhDMDH in the biosynthesis of PGA | Schleiferilactobacillus harbinensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (R)-mandelate | substrate inhibition | Schleiferilactobacillus harbinensis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
NAD+ | recombinant enzyme, pH 9.5, 30°C | Schleiferilactobacillus harbinensis | |
| 1.05 | - |
(R)-mandelate | recombinant enzyme, pH 9.5, 30°C | Schleiferilactobacillus harbinensis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phenylglyoxylate + NADH + H+ | Schleiferilactobacillus harbinensis | - |
(R)-mandelate + NAD+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schleiferilactobacillus harbinensis | A0A5P8Q188 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography | Schleiferilactobacillus harbinensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1200 | - |
recombinant enzyme, substrate (R)-mandelate, pH 9.5, 30°C | Schleiferilactobacillus harbinensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-mandelate + NAD+ | - |
Schleiferilactobacillus harbinensis | phenylglyoxylate + NADH + H+ | - |
r | |
| additional information | D-2-chloromandelic acid and L-mandelic acid are poor substrates, while L-2-chloromandelic acid and L-lactic acid are no substrates for enzyme LhDMDH | Schleiferilactobacillus harbinensis | ? | - |
- |
|
| phenylglyoxylate + NADH + H+ | - |
Schleiferilactobacillus harbinensis | (R)-mandelate + NAD+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, recombinant His-tagged enzyme, SDS-PAGE | Schleiferilactobacillus harbinensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-mandelate dehydrogenase | - |
Schleiferilactobacillus harbinensis |
| DMDH | - |
Schleiferilactobacillus harbinensis |
| LhDMDH | - |
Schleiferilactobacillus harbinensis |
| NAD+-dependent D-mandelate dehydrogenase | - |
Schleiferilactobacillus harbinensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
recombinant enzyme | Schleiferilactobacillus harbinensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | over 50% of maximal activity within this range, profile overview | Schleiferilactobacillus harbinensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
purified recombinant enzyme, residual activity of LhDMDH is less than 50% after being incubated at 30°C for 1 h | Schleiferilactobacillus harbinensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 29.05 | - |
NAD+ | recombinant enzyme, pH 9.5, 30°C | Schleiferilactobacillus harbinensis | |
| 30.28 | - |
(R)-mandelate | recombinant enzyme, pH 9.5, 30°C | Schleiferilactobacillus harbinensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
recombinant enzyme, oxidation of D-mandelic acid | Schleiferilactobacillus harbinensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
purified recombinant enzyme, 1 h at 0°C, 20% activity remaining | Schleiferilactobacillus harbinensis |
| 8.5 | - |
purified recombinant enzyme, 1 h at 0°C, 40% activity remaining | Schleiferilactobacillus harbinensis |
| 9 | - |
purified recombinant enzyme, 1 h at 0°C, 70% activity remaining | Schleiferilactobacillus harbinensis |
| 10.5 | - |
purified recombinant enzyme, 1 h at 0°C, completely stable at | Schleiferilactobacillus harbinensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity is detected with NADP+ as cofactor | Schleiferilactobacillus harbinensis | |
| NAD+ | - |
Schleiferilactobacillus harbinensis | |
| NADH | - |
Schleiferilactobacillus harbinensis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26.4 | - |
(R)-mandelate | recombinant enzyme, pH 9.5, 30°C | Schleiferilactobacillus harbinensis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 28.84 | - |
(R)-mandelate | recombinant enzyme, pH 9.5, 30°C | Schleiferilactobacillus harbinensis | |
| 726.25 | - |
NAD+ | recombinant enzyme, pH 9.5, 30°C | Schleiferilactobacillus harbinensis | |
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