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Literature summary for 1.1.1.379 extracted from

  • Baker, D.P.; Fewson, C.A.
    Purification and characterization of D(-)-mandelate dehydrogenase from Rhodotorula graminis (1989), Microbiology, 135, 2035-2044.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
(S)-Mandelate competitive Rhodotorula graminis
2-hydroxy-2-(4-hydroxyphenyl)propanoate 5 mM, 44.2% inhibition Rhodotorula graminis
2-hydroxy-3-methylbutanoate 10 mM, 31.9% inhibition Rhodotorula graminis
2-Hydroxy-4-methylpentanoate 1 mM, 55.1% inhibition Rhodotorula graminis
2-hydroxyhexanoate 1 mM, 63.8% inhibition Rhodotorula graminis
2-hydroxypentanoate 10 mM, 67.4% inhibition Rhodotorula graminis
2-oxo-3-methylbutanoate 10 mM, 43.1% inhibition Rhodotorula graminis
2-oxo-3-methylpentanoate 5 mM, 54.1% inhibition Rhodotorula graminis
2-oxo-4-methylpentanoate 1 mM, 40.4% inhibition Rhodotorula graminis
2-oxoglutarate 10 mM, 77.1% inhibition Rhodotorula graminis
2-Oxohexanoate 10 mM, 44.1% inhibition Rhodotorula graminis
2-oxooctanoate 2.5 mM, 28.5% inhibition Rhodotorula graminis
2-oxopentanoate 10 mM, 44.1% inhibition Rhodotorula graminis
D-lactate 10 mM, 17.4% inhibition Rhodotorula graminis
additional information neither stimulated nor inhibited by 1 mM concentrations of NaCl, Na2SO4, KCl, MgCl2, NH4Cl, CaCl2, ZnCl2, CoCl2, FeCl3, KH2PO4, NaH2PO4, MnSO4, MnCl2 or CuSO4. Not inhibited by the following metal-chelating agents each at 1 mM: EDTA, diethyldithiocarbamic acid, bathophenanthroline disulfonic acid, dihydroxybenzene disulfonic acid, pyrazole, 8-hydroxyquinoline or 2,2'-dipyridyl. Little or no inhibition of enzyme activity by 10 mM iodoacetate, 10 mM iodoacetamide, 6.65 mM 5,5'-dithiobis(2-nitrobenzoic acid) or 5 mM 4-chloromercuribenzoate, even after a 6 h preincubation at 27°C. Neither inhibited nor stimulated by 1 mM concentrations of benzaldehyde, benzoate, succinate, glucose, ATP/Mg2+, ADP/Mg2+ or acetyl-CoA Rhodotorula graminis
N-ethylmaleimide 10 mM, strong inhibition Rhodotorula graminis
oxalate 10 mM, 63.1% inhibition Rhodotorula graminis
oxaloacetate 10 mM, 38.5% inhibition Rhodotorula graminis
oxamate 10 mM,% inhibition Rhodotorula graminis
phenyl 2-hydroxypropanoate 10 mM, 47.1% inhibition Rhodotorula graminis
pyruvate 10 mM, 17.4% inhibition Rhodotorula graminis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information at all pH values tested, the apparent Km-values for NADH and phenylglyoxylate are significantly lower than for NAD+ and (R)-mandelate Rhodotorula graminis
0.026
-
NADH pH 9.5, 27°C Rhodotorula graminis
0.036
-
NADH pH 7.0, 27°C Rhodotorula graminis
0.038
-
NADH pH 5.9, 27°C Rhodotorula graminis
0.0455
-
phenylglyoxylate pH 7.0, 27°C Rhodotorula graminis
0.0515
-
phenylglyoxylate pH 5.9, 27°C Rhodotorula graminis
0.0707
-
phenylglyoxylate pH 9.5, 27°C Rhodotorula graminis
0.0775
-
NAD+ pH 9.5, 27°C Rhodotorula graminis
0.309
-
NAD+ pH 7.0, 27°C Rhodotorula graminis
0.325
-
(R)-mandelate pH 9.5, 27°C Rhodotorula graminis
3.05
-
(R)-mandelate pH 7.0, 27°C Rhodotorula graminis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information neither stimulated nor inhibited by 1 mM concentrations of NaCl, Na2SO4, KCl, MgCl2, NH4Cl, CaCl2, ZnCl2, CoCl2, FeCl3, KH2PO4, NaH2PO4, MnSO4, MnCl2, or CuSO4 Rhodotorula graminis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
2 * 38000, SDS-PAGE Rhodotorula graminis
77000
-
gel filtration Rhodotorula graminis

Organism

Organism UniProt Comment Textmining
Rhodotorula graminis Q7LLW9
-
-
Rhodotorula graminis KGX 39 Q7LLW9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rhodotorula graminis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
67.6
-
pH 9.5, 27°C, substrate: (R)-mandelate, purified enzyme Rhodotorula graminis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-mandelate + NAD+ equilibrium constant is 1.59 x 10(-11) M, pH 9.2 Rhodotorula graminis phenylglyoxylate + NADH + H+
-
r
(R)-mandelate + NAD+ equilibrium constant is 1.59 x 10(-11) M, pH 9.2 Rhodotorula graminis KGX 39 phenylglyoxylate + NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 38000, SDS-PAGE Rhodotorula graminis

Synonyms

Synonyms Comment Organism
D(-)-mandelate dehydrogenase
-
Rhodotorula graminis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.9
-
reduction of phenylglyoxylate Rhodotorula graminis
9.5
-
oxidation of R-mandelate Rhodotorula graminis

Cofactor

Cofactor Comment Organism Structure
NAD+ no activity with NADP+, 2,6-dichloroindophenol or 2,6-dichloroindophenol plus N-methylphenazonium methosulfate as electron acceptors Rhodotorula graminis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
8.2
-
(S)-Mandelate pH 9.5, 27°C Rhodotorula graminis

pI Value

Organism Comment pI Value Maximum pI Value
Rhodotorula graminis isoelectric focusing
-
5.9