Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-Mandelate | competitive | Rhodotorula graminis | |
2-hydroxy-2-(4-hydroxyphenyl)propanoate | 5 mM, 44.2% inhibition | Rhodotorula graminis | |
2-hydroxy-3-methylbutanoate | 10 mM, 31.9% inhibition | Rhodotorula graminis | |
2-Hydroxy-4-methylpentanoate | 1 mM, 55.1% inhibition | Rhodotorula graminis | |
2-hydroxyhexanoate | 1 mM, 63.8% inhibition | Rhodotorula graminis | |
2-hydroxypentanoate | 10 mM, 67.4% inhibition | Rhodotorula graminis | |
2-oxo-3-methylbutanoate | 10 mM, 43.1% inhibition | Rhodotorula graminis | |
2-oxo-3-methylpentanoate | 5 mM, 54.1% inhibition | Rhodotorula graminis | |
2-oxo-4-methylpentanoate | 1 mM, 40.4% inhibition | Rhodotorula graminis | |
2-oxoglutarate | 10 mM, 77.1% inhibition | Rhodotorula graminis | |
2-Oxohexanoate | 10 mM, 44.1% inhibition | Rhodotorula graminis | |
2-oxooctanoate | 2.5 mM, 28.5% inhibition | Rhodotorula graminis | |
2-oxopentanoate | 10 mM, 44.1% inhibition | Rhodotorula graminis | |
D-lactate | 10 mM, 17.4% inhibition | Rhodotorula graminis | |
additional information | neither stimulated nor inhibited by 1 mM concentrations of NaCl, Na2SO4, KCl, MgCl2, NH4Cl, CaCl2, ZnCl2, CoCl2, FeCl3, KH2PO4, NaH2PO4, MnSO4, MnCl2 or CuSO4. Not inhibited by the following metal-chelating agents each at 1 mM: EDTA, diethyldithiocarbamic acid, bathophenanthroline disulfonic acid, dihydroxybenzene disulfonic acid, pyrazole, 8-hydroxyquinoline or 2,2'-dipyridyl. Little or no inhibition of enzyme activity by 10 mM iodoacetate, 10 mM iodoacetamide, 6.65 mM 5,5'-dithiobis(2-nitrobenzoic acid) or 5 mM 4-chloromercuribenzoate, even after a 6 h preincubation at 27°C. Neither inhibited nor stimulated by 1 mM concentrations of benzaldehyde, benzoate, succinate, glucose, ATP/Mg2+, ADP/Mg2+ or acetyl-CoA | Rhodotorula graminis | |
N-ethylmaleimide | 10 mM, strong inhibition | Rhodotorula graminis | |
oxalate | 10 mM, 63.1% inhibition | Rhodotorula graminis | |
oxaloacetate | 10 mM, 38.5% inhibition | Rhodotorula graminis | |
oxamate | 10 mM,% inhibition | Rhodotorula graminis | |
phenyl 2-hydroxypropanoate | 10 mM, 47.1% inhibition | Rhodotorula graminis | |
pyruvate | 10 mM, 17.4% inhibition | Rhodotorula graminis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | at all pH values tested, the apparent Km-values for NADH and phenylglyoxylate are significantly lower than for NAD+ and (R)-mandelate | Rhodotorula graminis | |
0.026 | - |
NADH | pH 9.5, 27°C | Rhodotorula graminis | |
0.036 | - |
NADH | pH 7.0, 27°C | Rhodotorula graminis | |
0.038 | - |
NADH | pH 5.9, 27°C | Rhodotorula graminis | |
0.0455 | - |
phenylglyoxylate | pH 7.0, 27°C | Rhodotorula graminis | |
0.0515 | - |
phenylglyoxylate | pH 5.9, 27°C | Rhodotorula graminis | |
0.0707 | - |
phenylglyoxylate | pH 9.5, 27°C | Rhodotorula graminis | |
0.0775 | - |
NAD+ | pH 9.5, 27°C | Rhodotorula graminis | |
0.309 | - |
NAD+ | pH 7.0, 27°C | Rhodotorula graminis | |
0.325 | - |
(R)-mandelate | pH 9.5, 27°C | Rhodotorula graminis | |
3.05 | - |
(R)-mandelate | pH 7.0, 27°C | Rhodotorula graminis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | neither stimulated nor inhibited by 1 mM concentrations of NaCl, Na2SO4, KCl, MgCl2, NH4Cl, CaCl2, ZnCl2, CoCl2, FeCl3, KH2PO4, NaH2PO4, MnSO4, MnCl2, or CuSO4 | Rhodotorula graminis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Rhodotorula graminis |
77000 | - |
gel filtration | Rhodotorula graminis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula graminis | Q7LLW9 | - |
- |
Rhodotorula graminis KGX 39 | Q7LLW9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodotorula graminis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
67.6 | - |
pH 9.5, 27°C, substrate: (R)-mandelate, purified enzyme | Rhodotorula graminis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mandelate + NAD+ | equilibrium constant is 1.59 x 10(-11) M, pH 9.2 | Rhodotorula graminis | phenylglyoxylate + NADH + H+ | - |
r | |
(R)-mandelate + NAD+ | equilibrium constant is 1.59 x 10(-11) M, pH 9.2 | Rhodotorula graminis KGX 39 | phenylglyoxylate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Rhodotorula graminis |
Synonyms | Comment | Organism |
---|---|---|
D(-)-mandelate dehydrogenase | - |
Rhodotorula graminis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | - |
reduction of phenylglyoxylate | Rhodotorula graminis |
9.5 | - |
oxidation of R-mandelate | Rhodotorula graminis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | no activity with NADP+, 2,6-dichloroindophenol or 2,6-dichloroindophenol plus N-methylphenazonium methosulfate as electron acceptors | Rhodotorula graminis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
8.2 | - |
(S)-Mandelate | pH 9.5, 27°C | Rhodotorula graminis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodotorula graminis | isoelectric focusing | - |
5.9 |