BRENDA - Enzyme Database show
show all sequences of 1.1.1.374

Enzymatic analysis of uridine diphosphate N-acetyl-D-glucosamine

Namboori, S.C.; Graham, D.E.; Anal. Biochem. 381, 94-100 (2008)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
high-throughput chromatographic analysis of UDPGlcNAc in a complex matrix of deproteinized cell extract
Methanococcus maripaludis
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli BL21(DE3)
Methanococcus maripaludis
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
N-acetyl-D-glucosamine, D-glucose, and D-glucosamine do not inhibit the reaction at 3-mM concentrations
Methanococcus maripaludis
UDP
2 mM, reduces activity by 50%
Methanococcus maripaludis
UDP-alpha-D-glucose
2 mM, reduces activity by 50%
Methanococcus maripaludis
UDP-alpha-N-acetyl-D-galactosamine
3 mM, reduces activity by 50%
Methanococcus maripaludis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.18
-
NAD+
pH 8.5, 37C
Methanococcus maripaludis
0.18
-
UDP-N-acetyl-alpha-D-glucosamine
pH 8.5, 37C
Methanococcus maripaludis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
activity is 85% lower in the absence of KCl as compared with reactions containing 200 mM KCl
Methanococcus maripaludis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
8 * 39000, SDS-PAGE
Methanococcus maripaludis
305000
-
gel filtration
Methanococcus maripaludis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-alpha-D-glucosamine + NAD+
Methanococcus maripaludis
the enzyme is involved in the biosynthesis of glycan component sugars
UDP-2-acetamido-3-dehydro-2-deoxy-alpha-D-glucopyranose + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanococcus maripaludis
Q6M0B9
-
-
Purification (Commentary)
Commentary
Organism
-
Methanococcus maripaludis
Storage Stability
Storage Stability
Organism
-80C, stable for at least several weeks in 20% glycerol
Methanococcus maripaludis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-alpha-D-glucosamine + NAD+
the enzyme is involved in the biosynthesis of glycan component sugars
726671
Methanococcus maripaludis
UDP-2-acetamido-3-dehydro-2-deoxy-alpha-D-glucopyranose + NADH + H+
-
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + NAD+
no activity is detected when NAD+ is replaced with NADP+. The MMP0352 protein does not catalyze the oxidation of the substrate analogs UDPGlc, UDPGalNAc, N-acetylglucosamine, and glucosamine
726671
Methanococcus maripaludis
UDP-2-acetamido-3-dehydro-2-deoxy-alpha-D-glucopyranose + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
octamer
8 * 39000, SDS-PAGE
Methanococcus maripaludis
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Methanococcus maripaludis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.9
-
UDP-N-acetyl-alpha-D-glucosamine
pH 8.5, 37C
Methanococcus maripaludis
0.99
-
NAD+
pH 8.5, 37C
Methanococcus maripaludis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Methanococcus maripaludis
Application (protein specific)
Application
Commentary
Organism
analysis
high-throughput chromatographic analysis of UDPGlcNAc in a complex matrix of deproteinized cell extract
Methanococcus maripaludis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli BL21(DE3)
Methanococcus maripaludis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
N-acetyl-D-glucosamine, D-glucose, and D-glucosamine do not inhibit the reaction at 3-mM concentrations
Methanococcus maripaludis
UDP
2 mM, reduces activity by 50%
Methanococcus maripaludis
UDP-alpha-D-glucose
2 mM, reduces activity by 50%
Methanococcus maripaludis
UDP-alpha-N-acetyl-D-galactosamine
3 mM, reduces activity by 50%
Methanococcus maripaludis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.18
-
NAD+
pH 8.5, 37C
Methanococcus maripaludis
0.18
-
UDP-N-acetyl-alpha-D-glucosamine
pH 8.5, 37C
Methanococcus maripaludis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
activity is 85% lower in the absence of KCl as compared with reactions containing 200 mM KCl
Methanococcus maripaludis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
8 * 39000, SDS-PAGE
Methanococcus maripaludis
305000
-
gel filtration
Methanococcus maripaludis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-alpha-D-glucosamine + NAD+
Methanococcus maripaludis
the enzyme is involved in the biosynthesis of glycan component sugars
UDP-2-acetamido-3-dehydro-2-deoxy-alpha-D-glucopyranose + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methanococcus maripaludis
Storage Stability (protein specific)
Storage Stability
Organism
-80C, stable for at least several weeks in 20% glycerol
Methanococcus maripaludis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-alpha-D-glucosamine + NAD+
the enzyme is involved in the biosynthesis of glycan component sugars
726671
Methanococcus maripaludis
UDP-2-acetamido-3-dehydro-2-deoxy-alpha-D-glucopyranose + NADH + H+
-
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + NAD+
no activity is detected when NAD+ is replaced with NADP+. The MMP0352 protein does not catalyze the oxidation of the substrate analogs UDPGlc, UDPGalNAc, N-acetylglucosamine, and glucosamine
726671
Methanococcus maripaludis
UDP-2-acetamido-3-dehydro-2-deoxy-alpha-D-glucopyranose + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
octamer
8 * 39000, SDS-PAGE
Methanococcus maripaludis
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Methanococcus maripaludis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.9
-
UDP-N-acetyl-alpha-D-glucosamine
pH 8.5, 37C
Methanococcus maripaludis
0.99
-
NAD+
pH 8.5, 37C
Methanococcus maripaludis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Methanococcus maripaludis
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in the biosynthesis of glycan component sugars
Methanococcus maripaludis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in the biosynthesis of glycan component sugars
Methanococcus maripaludis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
5
-
UDP-N-acetyl-alpha-D-glucosamine
pH 8.5, 37C
Methanococcus maripaludis
5.5
-
NAD+
pH 8.5, 37C
Methanococcus maripaludis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
5
-
UDP-N-acetyl-alpha-D-glucosamine
pH 8.5, 37C
Methanococcus maripaludis
5.5
-
NAD+
pH 8.5, 37C
Methanococcus maripaludis
Other publictions for EC 1.1.1.374
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726671
Namboori
Enzymatic analysis of uridine ...
Methanococcus maripaludis
Anal. Biochem.
381
94-100
2008
-
1
1
-
-
-
4
2
-
1
2
1
-
4
-
-
1
-
-
-
-
1
2
1
1
-
-
2
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
4
-
2
-
1
2
1
-
-
-
1
-
-
-
1
2
1
1
-
-
2
1
-
-
-
-
1
1
-
2
2